Extracellular DNA as a Prognostic and Therapeutic Target in Mouse Colitis under DNase I Treatment
Language English Country Czech Republic Media print
Document type Journal Article
PubMed
29871733
DOI
10.14712/fb2018064010010
PII: file/5862/fb2018a0002.pdf
Knihovny.cz E-resources
- MeSH
- Biomarkers metabolism MeSH
- Molecular Targeted Therapy * MeSH
- Deoxyribonuclease I therapeutic use MeSH
- DNA metabolism MeSH
- Colitis drug therapy pathology MeSH
- Mice, Inbred C57BL MeSH
- Mice MeSH
- Prognosis MeSH
- Animals MeSH
- Check Tag
- Male MeSH
- Mice MeSH
- Animals MeSH
- Publication type
- Journal Article MeSH
- Names of Substances
- Biomarkers MeSH
- Deoxyribonuclease I MeSH
- DNA MeSH
The aim of this study was to investigate the potential of extracellular DNA as a prognostic and/or therapeutic target in inflammatory bowel disease. Fifty male C57BL/6J mice were used in the experiment. Acute colitis was induced by intake of 2% dextran sulphate sodium (DSS) for seven days followed by three days of water intake. DNase I was injected intravenously on days 3 and 7. Plasmatic levels of extracellular DNA (ecDNA) were measured on days 6 and 10. Weight loss, stool consistency and liquid intake were monitored throughout the experiment. Colon length and weight, myeloperoxidase activity and tumour necrosis factor α (TNF-α) levels were measured at sacrifice. DSS-treated mice displayed severe colitis, as shown by disease activity parameters. Both groups with colitis (DNase treated and untreated) had significantly poorer weight loss, colon length and stool consistency compared with control groups on water. No differences between the DNasetreated and untreated DSS groups were recorded. Myeloperoxidase activity and levels of TNF-α in colonic tissue were notably greater in both groups with colitis compared to controls. In addition, both biochemical markers were improved in the DNasetreated group with colitis compared to the untreated group. Although the disease activity was proved by several independent parameters in both groups with colitis, levels of ecDNA did not show any difference between the groups throughout or at the end of experiment. The role of ecDNA in experimental colitis has not been confirmed. However, DNase I injection resulted in some improvement, and thus should be studied in more detail.
References provided by Crossref.org
Sex Difference in Plasma Deoxyribonuclease Activity in Rats
Extracellular DNA Correlates with Intestinal Inflammation in Chemically Induced Colitis in Mice
