The capacity of cells and organisms to sustain, and to eventually adapt to, environmental and genetic insults declines with age. Because macroautophagy/autophagy is regarded as one of the major determinants of cellular fitness in vitro and in vivo, maneuvers that aim at promoting autophagy may slow down aging and promote health span. Caloric restriction (CR), a reduction in caloric intake without malnutrition, efficiently counteracts aging-associated features, yet is difficult to be applied to humans. Caloric-restriction mimetics (CRMs) are pharmacological agents that recapitulate the main biochemical properties of CR, namely a global reduction of protein acetylation and the induction of autophagy. We found that the ancient drug aspirin and its active metabolite salicylate stimulate autophagic flux by virtue of their inhibitory action on acetyltransferase EP300. The inhibition of EP300 results from a direct competition between salicylate and acetyl coenzyme A for binding to the catalytic domain of the enzyme. This mode of action appears to be conserved across evolution as it accounts for the induction of autophagy by aspirin in various mouse models and in the nematode Caenorhabditis elegans. In sum, aspirin acts as a CRM.

b INSERM U1138 Paris France

c Equipe 11 labellisée par la Ligue Nationale contre le Cancer Centre de Recherche des Cordeliers Paris France

e Université Pierre and Marie Curie Paris France

f Faculté de Médecine Université Paris Sud Paris XI Kremlin Bicêtre France

g Sotio a c Prague Czech Republic

Gustave Roussy Cancer Campus Villejuif France

h Metabolomics and Cell Biology Platforms Gustave Roussy Cancer Campus Villejuif France

i Pôle de Biologie Hôpital Européen Georges Pompidou AP HP Karolinska Institute Paris France

j Department of Women's and Children's Health Karolinska University Hospital Stockholm Sweden

Université Paris Descartes Paris 5 Sorbonne Paris Cité Paris France

Cell Rep. 2018 Feb 27;22(9):2395-2407. doi: 10.1016/j.celrep.2018.02.024. PubMed

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