Silver pyridine-2-sulfonate complex - its characterization, DNA binding, topoisomerase I inhibition, antimicrobial and anticancer response
Language English Country United States Media print-electronic
Document type Journal Article, Research Support, Non-U.S. Gov't
PubMed
29960924
DOI
10.1016/j.jinorgbio.2018.06.006
PII: S0162-0134(18)30193-4
Knihovny.cz E-resources
- Keywords
- Anticancer, Antimicrobial, Intercalation, Silver sulfadiazine, Silver sulfonate, Topoisomerase I inhibition,
- MeSH
- Anti-Bacterial Agents * chemical synthesis chemistry pharmacology MeSH
- Apoptosis drug effects MeSH
- Bacteria growth & development MeSH
- Topoisomerase I Inhibitors * chemical synthesis chemistry pharmacology MeSH
- Coordination Complexes * chemical synthesis chemistry pharmacology MeSH
- Microbial Sensitivity Tests MeSH
- Mice MeSH
- Cell Line, Tumor MeSH
- Pyridines * chemistry pharmacology MeSH
- Silver * chemistry pharmacology MeSH
- Cell Survival MeSH
- Animals MeSH
- Check Tag
- Mice MeSH
- Animals MeSH
- Publication type
- Journal Article MeSH
- Research Support, Non-U.S. Gov't MeSH
- Names of Substances
- Anti-Bacterial Agents * MeSH
- Topoisomerase I Inhibitors * MeSH
- Coordination Complexes * MeSH
- Pyridines * MeSH
- Silver * MeSH
In the current study the ability of silver pyridine-2-sulfonate complex to exert multiple biological activities is compared with the pharmacological action of silver sulfadiazine (AgSD). Polymeric form of {[Ag(py-2-SO3)]}n (AgPS) was synthesized and characterized by analytical techniques (IR, CHN, TG/DTA, MS) and its molecular formula was established. The crystal structure was determined by X-ray diffraction method and the polymeric complex crystallizes in the triclinic P-1 space group. The stability of Ag(I) complex was verified by 1H and 13C NMR measurements and the interaction with calf thymus DNA through UV-VIS and fluorescence quenching experiments was studied. The Ag(I) complex was able to interact with DNA by dual binding mode: partial intercalation along groove binding. The binding constants were calculated to be in the order of 103 M-1. Topoisomerase I inhibition study have shown that silver complex is inhibiting its activity at concentration of 30 μM. The cytotoxic activity of AgPS and AgSD against mouse leukaemia L1210 S, R and T cell line was also evaluated. AgPS showed higher cytotoxicity than AgSD after 48 h incubation. The results suggest that mechanism of cell death is necrosis with a contribution of late apoptosis. Antimicrobial testing indicates higher growth inhibition effect of AgPS with comparison to commercially available AgSD.
References provided by Crossref.org
Antimicrobial and Anticancer Application of Silver(I) Dipeptide Complexes
