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Protective role of antifreeze proteins on sterlet (Acipenser ruthenus) sperm during cryopreservation

Xin, Miaomiao
Author Xin, Miaomiao Faculty of Fisheries and Protection of Waters, South Bohemian Research Center of Aquaculture and Biodiversity of Hydrocenoses, Research Institute of Fish Culture and Hydrobiology, University of South Bohemia in Ceske Budejovice, Zatisi 728/II, 389 25, Vodnany, Czech Republic. mxin@frov.jcu.cz Sino-Czech Joint Laboratory of Fish Conservation and Biotechnology: Key Laboratory of Freshwater Biodiversity Conservation, Ministry of Agriculture of China, Yangtze River Fisheries Research Institute, Chinese Academy of Fishery Sciences, Wuhan, China. mxin@frov.jcu.cz
Sterba, Jan
Author Sterba, Jan Faculty of Science, University of South Bohemia in Ceske Budejovice, Branisovska 1760, 370 05, Ceske Budejovice, Czech Republic Biology Centre, Czech Academy of Sciences, Institute of Parasitology, Branisovska 31, 370 05, Ceske Budejovice, Czech Republic
Shaliutina-Kolesova, Anna
Author Shaliutina-Kolesova, Anna Faculty of Fisheries and Protection of Waters, South Bohemian Research Center of Aquaculture and Biodiversity of Hydrocenoses, Research Institute of Fish Culture and Hydrobiology, University of South Bohemia in Ceske Budejovice, Zatisi 728/II, 389 25, Vodnany, Czech Republic
Dzyuba, Borys
Author Dzyuba, Borys Faculty of Fisheries and Protection of Waters, South Bohemian Research Center of Aquaculture and Biodiversity of Hydrocenoses, Research Institute of Fish Culture and Hydrobiology, University of South Bohemia in Ceske Budejovice, Zatisi 728/II, 389 25, Vodnany, Czech Republic
Lieskovska, Jaroslava
Author Lieskovska, Jaroslava Faculty of Science, University of South Bohemia in Ceske Budejovice, Branisovska 1760, 370 05, Ceske Budejovice, Czech Republic Biology Centre, Czech Academy of Sciences, Institute of Parasitology, Branisovska 31, 370 05, Ceske Budejovice, Czech Republic
Boryshpolets, Serhii
Author Boryshpolets, Serhii Faculty of Fisheries and Protection of Waters, South Bohemian Research Center of Aquaculture and Biodiversity of Hydrocenoses, Research Institute of Fish Culture and Hydrobiology, University of South Bohemia in Ceske Budejovice, Zatisi 728/II, 389 25, Vodnany, Czech Republic
Siddique, Mohammad Abdul Momin
Author Siddique, Mohammad Abdul Momin Faculty of Fisheries and Protection of Waters, South Bohemian Research Center of Aquaculture and Biodiversity of Hydrocenoses, Research Institute of Fish Culture and Hydrobiology, University of South Bohemia in Ceske Budejovice, Zatisi 728/II, 389 25, Vodnany, Czech Republic Department of Oceanography, Noakhali Science and Technology University, Noakhali, 3814, Bangladesh
Kholodnyy, Vitaliy
Author Kholodnyy, Vitaliy Faculty of Fisheries and Protection of Waters, South Bohemian Research Center of Aquaculture and Biodiversity of Hydrocenoses, Research Institute of Fish Culture and Hydrobiology, University of South Bohemia in Ceske Budejovice, Zatisi 728/II, 389 25, Vodnany, Czech Republic
Lebeda, Ievgen
Author Lebeda, Ievgen Faculty of Fisheries and Protection of Waters, South Bohemian Research Center of Aquaculture and Biodiversity of Hydrocenoses, Research Institute of Fish Culture and Hydrobiology, University of South Bohemia in Ceske Budejovice, Zatisi 728/II, 389 25, Vodnany, Czech Republic
Linhart, Otomar
Author Linhart, Otomar Faculty of Fisheries and Protection of Waters, South Bohemian Research Center of Aquaculture and Biodiversity of Hydrocenoses, Research Institute of Fish Culture and Hydrobiology, University of South Bohemia in Ceske Budejovice, Zatisi 728/II, 389 25, Vodnany, Czech Republic

Fish physiology and biochemistry. 2018 Dec ; 44 (6) : 1527-1533. [epub] 20180718

Fish Physiol Biochem
ISSN 1573-5168 | 0920-1742
Source

Language English Country Netherlands Media print-electronic

Document type Journal Article

Persistent link https://www.medvik.cz/link/pmid30022341

PubMed 30022341
DOI 10.1007/s10695-018-0538-5
PII: 10.1007/s10695-018-0538-5
Knihovny.cz E-resources

Keywords
Antifreeze proteins, Cryopreservation, Membrane integrity, Motility rate, Sperm quality,
MeSH
Cryopreservation methods veterinary MeSH
Cryoprotective Agents pharmacology MeSH
Antifreeze Proteins pharmacology MeSH
Sperm Motility * MeSH
Fishes physiology MeSH
Semen Preservation methods veterinary MeSH
Animals MeSH
Check Tag
Male MeSH
Animals MeSH
Publication type
Journal Article MeSH
Names of Substances
Cryoprotective Agents MeSH
Antifreeze Proteins MeSH

The loss of sperm quality in sterlet (Acipenser ruthenus) due to freeze-thaw process in cryopreservation was investigated in the present study. Two antifreeze proteins (AFPI or AFPIII) were used at different concentrations of 0.1, 1, 10, and 100 μg/mL. We compared motility, curvilinear velocity, and plasma membrane integrity of fresh, cryopreserved sperm, and sperm cryopreserved in the presence of antifreeze proteins. Fresh sperm (control) had 85 ± 4% motility and 160 ± 2 μm/s curvilinear velocity, respectively. After cryopreservation, the motility of frozen-thawed sperm without addition of antifreeze proteins significantly decreased (44 ± 9%), compared to the control. The highest motility of frozen-thawed sperm was obtained in cryopreserved sperm with addition of 1 μg/mL of AFPIII (58 ± 14%). No significant differences were observed in curvilinear velocity between fresh sperm and cryopreserved sperm with/without addition of AFPI or AFPIII. The flow cytometry analysis revealed that fresh sperm contained 94.5 ± 6% live cells, while the cryopreserved sperm only contained 26.6 ± 14% live cells. Supplementation of antifreeze proteins has significantly improved the percentage of live cells in frozen-thawed sperm, except 0.1 μg/ml of AFPI group. No significant difference in percentage of live cells was detected in the sperm cryopreserved with 10 μg/mL of AFPI or AFPIII, compared to fresh sperm. Thus, addition of antifreeze proteins to cryopreservation medium could be considered to improve the post-thawed sperm quality of sterlet.

Biology Centre Czech Academy of Sciences Institute of Parasitology Branisovska 31 370 05 Ceske Budejovice Czech Republic

Department of Oceanography Noakhali Science and Technology University Noakhali 3814 Bangladesh

Faculty of Fisheries and Protection of Waters South Bohemian Research Center of Aquaculture and Biodiversity of Hydrocenoses Research Institute of Fish Culture and Hydrobiology University of South Bohemia in Ceske Budejovice Zatisi 728 2 389 25 Vodnany Czech Republic

Faculty of Science University of South Bohemia in Ceske Budejovice Branisovska 1760 370 05 Ceske Budejovice Czech Republic

Sino Czech Joint Laboratory of Fish Conservation and Biotechnology Key Laboratory of Freshwater Biodiversity Conservation Ministry of Agriculture of China Yangtze River Fisheries Research Institute Chinese Academy of Fishery Sciences Wuhan China

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