Photosynthetic and Stress Responsive Proteins Are Altered More Effectively in Nicotiana benthamiana Infected with Plum pox virus Aggressive PPV-CR versus Mild PPV-C Cherry-Adapted Isolates
Language English Country United States Media print-electronic
Document type Journal Article, Research Support, Non-U.S. Gov't
- Keywords
- LC−MS, antioxidants, compatible interaction, label-free quantification, photosynthesis, sharka, tobacco,
- MeSH
- Chlorophyll biosynthesis MeSH
- Chromatography, Reverse-Phase MeSH
- Energy Metabolism genetics MeSH
- Photosynthesis genetics MeSH
- Genotype MeSH
- Glutathione biosynthesis MeSH
- Mass Spectrometry MeSH
- Host-Pathogen Interactions genetics MeSH
- Carotenoids biosynthesis MeSH
- Plant Leaves genetics metabolism virology MeSH
- Plant Diseases genetics virology MeSH
- Oxidation-Reduction MeSH
- Hydrogen Peroxide metabolism MeSH
- Heat-Shock Proteins classification genetics metabolism MeSH
- Prunus avium virology MeSH
- Gene Expression Regulation, Plant * MeSH
- Plant Proteins classification genetics metabolism MeSH
- Prunus domestica virology MeSH
- Nicotiana genetics metabolism virology MeSH
- Plum Pox Virus classification genetics growth & development pathogenicity MeSH
- Chromatography, High Pressure Liquid MeSH
- Publication type
- Journal Article MeSH
- Research Support, Non-U.S. Gov't MeSH
- Names of Substances
- Chlorophyll MeSH
- Glutathione MeSH
- Carotenoids MeSH
- Hydrogen Peroxide MeSH
- Heat-Shock Proteins MeSH
- Plant Proteins MeSH
Plum pox virus (PPV, family Potyviridae) is one of the most important viral pathogens of Prunus spp. causing considerable damage to stone-fruit industry worldwide. Among the PPV strains identified so far, only PPV-C, PPV-CR, and PPV-CV are able to infect cherries under natural conditions. Herein, we evaluated the pathogenic potential of two viral isolates in herbaceous host Nicotiana benthamiana. Significantly higher accumulation of PPV capsid protein in tobacco leaves infected with PPV-CR (RU-30sc isolate) was detected in contrast to PPV-C (BY-101 isolate). This result correlated well with the symptoms observed in the infected plants. To further explore the host response upon viral infection at the molecular level, a comprehensive proteomic profiling was performed. Using reverse-phase ultra-high-performance liquid chromatography followed by label-free mass spectrometry quantification, we identified 38 unique plant proteins as significantly altered due to the infection. Notably, the abundances of photosynthesis-related proteins, mainly from the Calvin-Benson cycle, were found more aggressively affected in plants infected with PPV-CR isolate than those of PPV-C. This observation was accompanied by a significant reduction in the amount of photosynthetic pigments extracted from the leaves of PPV-CR infected plants. Shifts in the abundance of proteins that are involved in stimulation of photosynthetic capacity, modification of amino acid, and carbohydrate metabolism may affect plant growth and initiate energy formation via gluconeogenesis in PPV infected N. benthamiana. Furthermore, we suggest that the higher accumulation of H2O2 in PPV-CR infected leaves plays a crucial role in plant defense and development by activating the glutathione synthesis.
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