The uropygial gland of hoopoe nestlings and nesting females hosts bacterial symbionts that cause changes in the characteristics of its secretion, including an increase of its antimicrobial activity. These changes occur only in nesting individuals during the breeding season, possibly associated with the high infection risk experienced during the stay in the hole-nests. However, the knowledge on hoopoes uropygial gland microbial community dynamics is quite limited and based so far on culture-dependent and molecular fingerprinting studies. In this work, we sampled wild and captive hoopoes of different sex, age, and reproductive status, and studied their microbiota using quantitative polymerase chain reaction (qPCR), fluorescence in situ hybridization (FISH) and pyrosequencing. Surprisingly, we found a complex bacterial community in all individuals (including non-nesting ones) during the breeding season. Nevertheless, dark secretions from nesting hoopoes harbored significantly higher bacterial density than white secretions from breeding males and both sexes in winter. We hypothesize that bacterial proliferation may be host-regulated in phases of high infection risk (i.e., nesting). We also highlight the importance of specific antimicrobial-producing bacteria present only in dark secretions that may be key in this defensive symbiosis. Finally, we discuss the possible role of environmental conditions in shaping the uropygial microbiota, based on differences found between wild and captive hoopoes.

Departamento de Microbiología Universidad de Granada E 18071 Granada Spain

Departamento de Zoología Universidad de Granada E 18071 Granada Spain

Estación Experimental de Zonas Áridas E 04120 Almeria Spain

Faculty of Science University of South Bohemia CZ 370 05 Ceske Budejovice Czechia

Instituto de Biotecnología Universidad de Granada E 18071 Granada Spain

Unidad Asociada Coevolución Cucos Hospedadores y Bacterias Simbiontes Universidad de Granada E 18071 Granada Spain

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