Bioactive compounds present in plant-based foods, and their metabolites derived from gut microbiota and endogenous metabolism, represent thousands of chemical structures of potential interest for human nutrition and health. State-of-the-art analytical methodologies, including untargeted metabolomics based on high-resolution mass spectrometry, are required for the profiling of these compounds in complex matrices, including plant food materials and biofluids. The aim of this project was to compare the analytical coverage of untargeted metabolomics methods independently developed and employed in various European platforms. In total, 56 chemical standards representing the most common classes of bioactive compounds spread over a wide chemical space were selected and analyzed by the participating platforms (n = 13) using their preferred untargeted method. The results were used to define analytical criteria for a successful analysis of plant food bioactives. Furthermore, they will serve as a basis for an optimized consensus method.

Alkalmazott Kemia Tsz Elelmiszertudomanyi Kar Szent István Egyetem 29 43 Villanyi Street 1118 Budapest Hungary

Department of Food Science and Technology CEBAS CSIC Campus Universitario de Espinardo edf 25 30100 Murcia Spain

Department of Metabolism and Nutrition Institute of Food Science Technology and Nutrition Jose Antonio Novais 10 28040 Madrid Spain

Department of Nutrition Food Science and Gastronomy Pharmacy Faculty University of Barcelona Av Joan XXIII s n 08028 Barcelona Spain; CIBER Fragilidad y Envejecimiento Saludable Instituto de Salud Carlos 3 Barcelona Spain;

Division of Food and Nutrition Science Department of Biology and Biological Engineering Chalmers University of Technology SE 412 96 Gothenburg Sweden

INRA Human Nutrition Unit Université Clermont Auvergne F63000 Clermont Ferrand France

Institute of Animal Reproduction and Food Research of the Polish Academy of Sciences Tuwima 10 10 748 Olsztyn Poland

Institute of Public Health and Clinical Nutrition University of Eastern Finland P O Box 1627 FI 70211 Kuopio Finland

Instituto de Biologia Experimental Tecnológica Av da República 2780 157 Oeiras Portugal

Instituto de Tecnologia Química e Biológica Universidade Nova de Lisboa Av da República 2780 157 Oeiras Portugal

Laboratory of Biotransformation Institute of Microbiology of the CAS Vídeňská 1083 CZ 142 20 Prague Czechia

Research Institute for Medicines Avenida Professor Gama Pinto 1649 003 Lisbon Portugal

Technology and Food Science Department Flanders Research Institute for Agriculture Fisheries and Food Brusselsesteenweg 370 B 9090 Melle Belgium

VTT Technical Research Centre of Finland Ltd P O Box 1000 FI 02044 VTT Espoo Finland

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