Fractionation Techniques to Increase Plant Proteome Coverage: Combining Separation in Parallel at the Protein and the Peptide Level
Methods in molecular biology (Clifton, N.J.).
2019 ;
1871 () :
93-105.
Language English Country United States Media print
Document type Journal Article, Research Support, Non-U.S. Gov't
Persistent link
https://www.medvik.cz/link/pmid30276734
- Keywords
- C18, PEG, Peptide fractionation, Plant proteomics, Protein fractionation, SCX,
- MeSH
- Chemical Fractionation * methods MeSH
- Peptides analysis isolation & purification MeSH
- Proteome * MeSH
- Proteomics * methods MeSH
- Plant Proteins analysis isolation & purification MeSH
- Solvents MeSH
- Publication type
- Journal Article MeSH
- Research Support, Non-U.S. Gov't MeSH
- Names of Substances
- Peptides MeSH
- Proteome * MeSH
- Plant Proteins MeSH
- Solvents MeSH
Peptide spectral libraries enable targeted identification and quantitation of low-abundance proteins in a complex plant proteome. Here we describe parallel protein and peptide fractionation techniques to improve plant proteome coverage and facilitate construction of spectral libraries.
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