Amniotic fluid cell-free DNA in preterm prelabor rupture of membranes
Language English Country Great Britain, England Media print-electronic
Document type Journal Article, Research Support, Non-U.S. Gov't
Grant support
Charles University Prague, Faculty of Medicine in Hradec Kralove, Czech Republic - International
Faculty Hospital in Hradec Kralove - International
PubMed
30276834
DOI
10.1002/pd.5366
Knihovny.cz E-resources
- MeSH
- Amniocentesis MeSH
- Bacterial Infections metabolism MeSH
- Chlamydia trachomatis MeSH
- Chorioamnionitis metabolism MeSH
- Adult MeSH
- Gestational Age MeSH
- Interleukin-6 metabolism MeSH
- Cohort Studies MeSH
- Culture Techniques MeSH
- Real-Time Polymerase Chain Reaction MeSH
- Humans MeSH
- DNA, Mitochondrial metabolism MeSH
- Mycoplasma hominis MeSH
- Amniotic Fluid metabolism microbiology MeSH
- Polymerase Chain Reaction MeSH
- Fetal Membranes, Premature Rupture metabolism MeSH
- Retrospective Studies MeSH
- RNA, Ribosomal, 16S analysis MeSH
- Pregnancy MeSH
- Ureaplasma MeSH
- Cell-Free Nucleic Acids metabolism MeSH
- Inflammation metabolism MeSH
- Check Tag
- Adult MeSH
- Humans MeSH
- Pregnancy MeSH
- Female MeSH
- Publication type
- Journal Article MeSH
- Research Support, Non-U.S. Gov't MeSH
- Names of Substances
- IL6 protein, human MeSH Browser
- Interleukin-6 MeSH
- DNA, Mitochondrial MeSH
- RNA, Ribosomal, 16S MeSH
- Cell-Free Nucleic Acids MeSH
INTRODUCTION: We evaluated the levels of cell-free nuclear DNA (nDNA) and cell-free mitochondrial DNA (mtDNA) in the amniotic fluid supernatant from pregnancies complicated by preterm prelabor rupture of membranes (PPROM) based on evidence of microbial invasion of the amniotic cavity (MIAC) and/or intra-amniotic inflammation (IAI). MATERIAL AND METHODS: A total of 155 women with PPROM were included in this study. Amniotic fluid samples were obtained by transabdominal amniocentesis. The levels of cell-free nDNA and mtDNA in the amniotic fluid supernatant were assessed and quantified by real-time polymerase chain reaction. RESULTS: The levels of cell-free nDNA and mtDNA were higher in women with MIAC and IAI than in women without these conditions (nDNA: with MIAC: median 3.9 × 104 genome equivalent [GE]/mL vs without MIAC: median 1.2 × 104 GE/mL, with IAI: median: 5.3 × 104 GE/mL vs without IAI: median 1.2 × 104 GE/mL; mtDNA: with MIAC: median 9.2 × 105 GE/mL vs without MIAC: median 2.5 × 105 GE/mL, with IAI: median 1.1 × 106 GE/mL vs without IAI: median 2.5 × 105 ; all P values ≤ 0.01). Women with the microbial-associated IAI showed the highest levels of cell-free nDNA and mtDNA. CONCLUSIONS: Cell-free nDNA and mtDNA are constituents of the amniotic fluid supernatant from PPROM pregnancies. Both cell-free nDNA and mtDNA are involved in the intra-amniotic inflammatory response in women with PPROM.
Biomedical Research Center University Hospital Hradec Kralove Hradec Kralove Czech Republic
Department of Molecular Biology Faculty of Natural Sciences Comenius University Bratislava Slovakia
Department of Obstetrics and Gynecology Sahlgrenska Academy Gothenburg University Gothenburg Sweden
Institute of Molecular Biomedicine Faculty of Medicine Comenius University Bratislava Slovakia
Institute of Pathophysiology Faculty of Medicine Comenius University Bratislava Slovakia
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