The Complex Portal (www.ebi.ac.uk/complexportal) is a manually curated, encyclopaedic database that collates and summarizes information on stable, macromolecular complexes of known function. It captures complex composition, topology and function and links out to a large range of domain-specific resources that hold more detailed data, such as PDB or Reactome. We have made several significant improvements since our last update, including improving compliance to the FAIR data principles by providing complex-specific, stable identifiers that include versioning. Protein complexes are now available from 20 species for download in standards-compliant formats such as PSI-XML, MI-JSON and ComplexTAB or can be accessed via an improved REST API. A component-based JS front-end framework has been implemented to drive a new website and this has allowed the use of APIs from linked services to import and visualize information such as the 3D structure of protein complexes, its role in reactions and pathways and the co-expression of complex components in the tissues of multi-cellular organisms. A first draft of the complete complexome of Saccharomyces cerevisiae is now available to browse and download.

Chongqing Key Laboratory on Big Data for Bio Intelligence Chongqing University of Posts and Telecommunications No 2 Chongwen Road Nan'an District Chongqing 400065 China

Department of Biochemistry and National Centre for Biomolecular Research Faculty of Science Masaryk University Kamenice 5 625 00 Brno Bohunice Czech Republic

Department of Genetics Stanford University School of Medicine Stanford CA 94305 5477 USA

European Bioinformatics Institute European Molecular Biology Laboratory Wellcome Genome Campus Hinxton Cambridgeshire CB10 1SD UK

Novartis Institutes for BioMedical Research Basel Canton of Basel Stadt Switzerland

State Key Laboratory of Proteomics Beijing Proteome Research Center National Center for Protein Sciences Beijing Institute of Life Omics Beijing 102206 China

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