Using Amphioxus as a Basal Chordate Model to Study BMP Signaling Pathway
Language English Country United States Media print
Document type Journal Article, Research Support, Non-U.S. Gov't
- Keywords
- Amphioxus, BMP, Dorsomorphin, Embryo, Immunostaining, In situ hybridization,
- MeSH
- Models, Biological * MeSH
- Embryo, Nonmammalian MeSH
- Embryonic Development MeSH
- In Situ Hybridization, Fluorescence MeSH
- Lancelets metabolism MeSH
- Bone Morphogenetic Proteins metabolism pharmacology MeSH
- Recombinant Proteins pharmacology MeSH
- Signal Transduction * drug effects MeSH
- Animals MeSH
- Check Tag
- Animals MeSH
- Publication type
- Journal Article MeSH
- Research Support, Non-U.S. Gov't MeSH
- Names of Substances
- Bone Morphogenetic Proteins MeSH
- Recombinant Proteins MeSH
The BMP signaling pathway has been shown to be involved in different aspects of embryonic development across diverse metazoan phyla. Comparative studies on the roles of the BMP signaling pathway provide crucial insights into the evolution of the animal body plans. In this chapter, we present the general workflow on how to investigate the roles of BMP signaling pathway during amphioxus embryonic development. As amphioxus are basal invertebrate chordates, studies on the BMP signaling pathway in amphioxus could elucidate the functional evolution of BMP pathway in the chordate group. Here, we describe methods for animal husbandry, spawning induction, and manipulation of the BMP signaling pathway during embryonic development through drug inhibitors and recombinant proteins. We also introduce an efficient method of using mesh baskets to handle amphioxus embryos for fluorescence immunostaining and multicolor fluorescence in situ hybridization and to assay the effects of manipulating BMP signaling pathway during amphioxus embryogenesis.
Institute of Cellular and Organismic Biology Academia Sinica Taipei Taiwan
Institute of Molecular Genetics of the ASCR Prague Czech Republic
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