Using Amphioxus as a Basal Chordate Model to Study BMP Signaling Pathway
Jazyk angličtina Země Spojené státy americké Médium print
Typ dokumentu časopisecké články, práce podpořená grantem
- Klíčová slova
- Amphioxus, BMP, Dorsomorphin, Embryo, Immunostaining, In situ hybridization,
- MeSH
- biologické modely * MeSH
- embryo nesavčí MeSH
- embryonální vývoj MeSH
- hybridizace in situ fluorescenční MeSH
- kopinatci metabolismus MeSH
- kostní morfogenetické proteiny metabolismus farmakologie MeSH
- rekombinantní proteiny farmakologie MeSH
- signální transdukce * účinky léků MeSH
- zvířata MeSH
- Check Tag
- zvířata MeSH
- Publikační typ
- časopisecké články MeSH
- práce podpořená grantem MeSH
- Názvy látek
- kostní morfogenetické proteiny MeSH
- rekombinantní proteiny MeSH
The BMP signaling pathway has been shown to be involved in different aspects of embryonic development across diverse metazoan phyla. Comparative studies on the roles of the BMP signaling pathway provide crucial insights into the evolution of the animal body plans. In this chapter, we present the general workflow on how to investigate the roles of BMP signaling pathway during amphioxus embryonic development. As amphioxus are basal invertebrate chordates, studies on the BMP signaling pathway in amphioxus could elucidate the functional evolution of BMP pathway in the chordate group. Here, we describe methods for animal husbandry, spawning induction, and manipulation of the BMP signaling pathway during embryonic development through drug inhibitors and recombinant proteins. We also introduce an efficient method of using mesh baskets to handle amphioxus embryos for fluorescence immunostaining and multicolor fluorescence in situ hybridization and to assay the effects of manipulating BMP signaling pathway during amphioxus embryogenesis.
Institute of Cellular and Organismic Biology Academia Sinica Taipei Taiwan
Institute of Molecular Genetics of the ASCR Prague Czech Republic
Citace poskytuje Crossref.org
