Generation of human iPSCs from human prostate cancer-associated fibroblasts IBPi002-A

. 2018 Dec ; 33 () : 255-259. [epub] 20181116

Jazyk angličtina Země Velká Británie, Anglie Médium print-electronic

Typ dokumentu kazuistiky, časopisecké články, práce podpořená grantem

Perzistentní odkaz   https://www.medvik.cz/link/pmid30481741
Odkazy

PubMed 30481741
DOI 10.1016/j.scr.2018.11.006
PII: S1873-5061(18)30275-7
Knihovny.cz E-zdroje

A human induced pluripotent stem cell line was generated from cancer-associated fibroblasts of a 68-years old patient with diagnosed prostate adenocarcinoma (PCa). The fibroblast cell line was reprogrammed with Epi5™ Episomal iPSC Reprogramming Kit. Pluripotency of the derived transgene-free iPS cell line was confirmed both in vitro by detecting expression of factors of pluripotency on a single-cell level, and also in vivo using teratoma formation assay. This new iPS cell line may be used for differentiation into different prostate-specific cell types in differentiation studies.

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