Seroepidemiological and molecular investigations of infections with Crimean-Congo haemorrhagic fever virus in Kazakhstan
Jazyk angličtina Země Kanada Médium print-electronic
Typ dokumentu časopisecké články
PubMed
30522982
DOI
10.1016/j.ijid.2018.10.015
PII: S1201-9712(18)34561-2
Knihovny.cz E-zdroje
- Klíčová slova
- Crimean–Congo haemorrhagic fever virus, Fever of unknown origin, Kazakhstan, Reassortment,
- MeSH
- dospělí MeSH
- ELISA MeSH
- hemoragická horečka krymská komplikace epidemiologie MeSH
- horečka neznámého původu etiologie MeSH
- koně MeSH
- lidé středního věku MeSH
- lidé MeSH
- mladiství MeSH
- mladý dospělý MeSH
- protilátky virové krev MeSH
- RNA virová krev MeSH
- séroepidemiologické studie MeSH
- skot MeSH
- virus krymsko-konžské hemoragické horečky genetika imunologie MeSH
- zvířata MeSH
- Check Tag
- dospělí MeSH
- lidé středního věku MeSH
- lidé MeSH
- mladiství MeSH
- mladý dospělý MeSH
- mužské pohlaví MeSH
- skot MeSH
- ženské pohlaví MeSH
- zvířata MeSH
- Publikační typ
- časopisecké články MeSH
- Geografické názvy
- Kazachstán epidemiologie MeSH
- Názvy látek
- protilátky virové MeSH
- RNA virová MeSH
OBJECTIVES: The aim of this study was to detect the seroprevalence of Crimean-Congo haemorrhagic fever virus (CCHFV) in patients with fever of unknown origin (FUO) in endemic (Kyzylorda) and non-endemic (Almaty) oblasts of Kazakhstan. METHODS: Paired serum samples from 802 patients with FUO were collected. Serum samples were investigated by ELISA to detect IgG and IgM antibodies against CCHFV. Sera with suspected acute infection were further investigated by RT-PCR to detect the viral RNA. RESULTS: IgG antibodies were detected in 12.7% of the sera from both oblasts. Acute infection was shown by IgM ELISA in four patients from Kyzylorda, with only one developing severe CCHF. Viral RNA was found by RT-PCR in the other three patients' sera. Phylogenetic analysis of partial L and S segments revealed CCHFV genotype Asia 2 and a possible reassortment between the genotypes Asia 1/Asia 2. Animal husbandry, such as working with cattle and horses, was significantly associated with CCHFV seropositivity. CONCLUSIONS: The antibodies and viral RNA detected in sera indicate that mild or even asymptomatic CCHFV infections are presented in Kazakhstan. This study describes the circulation of CCHFV in the so far non-endemic Almaty oblast for the first time. In conclusion, physicians treating patients with FUO in Kazakhstan should be aware of mild CCHF.
Centre for International Health Ludwig Maximilian University Munich Germany
Faculty of Law Charles University Prague Czech Republic
German Association for International Cooperation Almaty Kazakhstan
Kazakh National Medical University Almaty Kazakhstan
Scientific Practical Centre for Sanitary Epidemiological Expertise and Monitoring Almaty Kazakhstan
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