Seroepidemiological and molecular investigations of infections with Crimean-Congo haemorrhagic fever virus in Kazakhstan
Language English Country Canada Media print-electronic
Document type Journal Article
PubMed
30522982
DOI
10.1016/j.ijid.2018.10.015
PII: S1201-9712(18)34561-2
Knihovny.cz E-resources
- Keywords
- Crimean–Congo haemorrhagic fever virus, Fever of unknown origin, Kazakhstan, Reassortment,
- MeSH
- Adult MeSH
- Enzyme-Linked Immunosorbent Assay MeSH
- Hemorrhagic Fever, Crimean complications epidemiology MeSH
- Fever of Unknown Origin etiology MeSH
- Horses MeSH
- Middle Aged MeSH
- Humans MeSH
- Adolescent MeSH
- Young Adult MeSH
- Antibodies, Viral blood MeSH
- RNA, Viral blood MeSH
- Seroepidemiologic Studies MeSH
- Cattle MeSH
- Hemorrhagic Fever Virus, Crimean-Congo genetics immunology MeSH
- Animals MeSH
- Check Tag
- Adult MeSH
- Middle Aged MeSH
- Humans MeSH
- Adolescent MeSH
- Young Adult MeSH
- Male MeSH
- Cattle MeSH
- Female MeSH
- Animals MeSH
- Publication type
- Journal Article MeSH
- Geographicals
- Kazakhstan epidemiology MeSH
- Names of Substances
- Antibodies, Viral MeSH
- RNA, Viral MeSH
OBJECTIVES: The aim of this study was to detect the seroprevalence of Crimean-Congo haemorrhagic fever virus (CCHFV) in patients with fever of unknown origin (FUO) in endemic (Kyzylorda) and non-endemic (Almaty) oblasts of Kazakhstan. METHODS: Paired serum samples from 802 patients with FUO were collected. Serum samples were investigated by ELISA to detect IgG and IgM antibodies against CCHFV. Sera with suspected acute infection were further investigated by RT-PCR to detect the viral RNA. RESULTS: IgG antibodies were detected in 12.7% of the sera from both oblasts. Acute infection was shown by IgM ELISA in four patients from Kyzylorda, with only one developing severe CCHF. Viral RNA was found by RT-PCR in the other three patients' sera. Phylogenetic analysis of partial L and S segments revealed CCHFV genotype Asia 2 and a possible reassortment between the genotypes Asia 1/Asia 2. Animal husbandry, such as working with cattle and horses, was significantly associated with CCHFV seropositivity. CONCLUSIONS: The antibodies and viral RNA detected in sera indicate that mild or even asymptomatic CCHFV infections are presented in Kazakhstan. This study describes the circulation of CCHFV in the so far non-endemic Almaty oblast for the first time. In conclusion, physicians treating patients with FUO in Kazakhstan should be aware of mild CCHF.
Centre for International Health Ludwig Maximilian University Munich Germany
Faculty of Law Charles University Prague Czech Republic
German Association for International Cooperation Almaty Kazakhstan
Kazakh National Medical University Almaty Kazakhstan
Scientific Practical Centre for Sanitary Epidemiological Expertise and Monitoring Almaty Kazakhstan
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