The degree of substitution affects the enantioselectivity of sulfobutylether-β-cyclodextrin chiral stationary phases
Jazyk angličtina Země Německo Médium print-electronic
Typ dokumentu časopisecké články, práce podpořená grantem
Grantová podpora
19-18005Y
Czech Science Foundation - International
PubMed
30671992
DOI
10.1002/elps.201800471
Knihovny.cz E-zdroje
- Klíčová slova
- Chromatography, Degree of substitution, Dynamic coating, Enantioseparation, Sulfobutylether-β-cyclodextrin,
- MeSH
- beta-cyklodextriny chemie MeSH
- chemické modely MeSH
- chromatografie s reverzní fází MeSH
- stereoizomerie MeSH
- vysokoúčinná kapalinová chromatografie metody MeSH
- Publikační typ
- časopisecké články MeSH
- práce podpořená grantem MeSH
- Názvy látek
- beta-cyklodextriny MeSH
- SBE4-beta-cyclodextrin MeSH Prohlížeč
Three chiral stationary phases were prepared by dynamic coating of sulfobutylether-β-cyclodextrin (SBE-β-CD) with different degrees of substitution, onto strong anion-exchange stationary phases. The enantioselective potential and stability of newly prepared chiral stationary phases were examined using a set of structurally different chiral analytes. Measurements were performed in RP-HPLC. Mobile phases consisted of methanol/formic acid, pH 2.10, and methanol/10 mM ammonium acetate buffer, pH 4.00, in various volume ratios. SBE-β-CDs with degrees of substitution (DS) 4, 6.3, and 10 proved suitable for the enantioseparation of 14, 11, and 8 analytes, respectively. The SBE-β-CD DS 4 based chiral stationary phase enabled the enantioseparation of the nearly all basic and neutral compounds. Chiral stationary phases with higher sulfobutylether-β-cyclodextrin substitution (especially DS 10) yielded higher enantioresolution values for acidic compounds.
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