The degree of substitution affects the enantioselectivity of sulfobutylether-β-cyclodextrin chiral stationary phases
Language English Country Germany Media print-electronic
Document type Journal Article, Research Support, Non-U.S. Gov't
Grant support
19-18005Y
Czech Science Foundation - International
- Keywords
- Chromatography, Degree of substitution, Dynamic coating, Enantioseparation, Sulfobutylether-β-cyclodextrin,
- MeSH
- beta-Cyclodextrins chemistry MeSH
- Models, Chemical MeSH
- Chromatography, Reverse-Phase MeSH
- Stereoisomerism MeSH
- Chromatography, High Pressure Liquid methods MeSH
- Publication type
- Journal Article MeSH
- Research Support, Non-U.S. Gov't MeSH
- Names of Substances
- beta-Cyclodextrins MeSH
- SBE4-beta-cyclodextrin MeSH Browser
Three chiral stationary phases were prepared by dynamic coating of sulfobutylether-β-cyclodextrin (SBE-β-CD) with different degrees of substitution, onto strong anion-exchange stationary phases. The enantioselective potential and stability of newly prepared chiral stationary phases were examined using a set of structurally different chiral analytes. Measurements were performed in RP-HPLC. Mobile phases consisted of methanol/formic acid, pH 2.10, and methanol/10 mM ammonium acetate buffer, pH 4.00, in various volume ratios. SBE-β-CDs with degrees of substitution (DS) 4, 6.3, and 10 proved suitable for the enantioseparation of 14, 11, and 8 analytes, respectively. The SBE-β-CD DS 4 based chiral stationary phase enabled the enantioseparation of the nearly all basic and neutral compounds. Chiral stationary phases with higher sulfobutylether-β-cyclodextrin substitution (especially DS 10) yielded higher enantioresolution values for acidic compounds.
References provided by Crossref.org
