Nuclear Genome Size in Contrast to Sex Chromosome Number Variability in the Human Bed Bug, Cimex lectularius (Heteroptera: Cimicidae)
Language English Country United States Media print-electronic
Document type Journal Article, Research Support, Non-U.S. Gov't
PubMed
30729668
DOI
10.1002/cyto.a.23729
Knihovny.cz E-resources
- Keywords
- Cimex lectularius, chromosome number variability, cytogenetics, flow cytometry, genome size, holokinetic chromosomes, internal standard,
- MeSH
- Staining and Labeling MeSH
- Cell Nucleus chemistry genetics MeSH
- Cytogenetics MeSH
- Genome Size * MeSH
- Indoles MeSH
- Meiosis genetics MeSH
- Metaphase genetics MeSH
- Mitosis genetics MeSH
- Sex Chromosomes * MeSH
- Propidium MeSH
- Flow Cytometry MeSH
- Bedbugs genetics MeSH
- Base Composition MeSH
- Animals MeSH
- Check Tag
- Male MeSH
- Female MeSH
- Animals MeSH
- Publication type
- Journal Article MeSH
- Research Support, Non-U.S. Gov't MeSH
- Names of Substances
- DAPI MeSH Browser
- Indoles MeSH
- Propidium MeSH
The human bed bug Cimex lectularius is one of the most prevalent human ectoparasites in temperate climate zones. The cytogenetic features of this resilient pest include holokinetic chromosomes, special chromosome behavior in meiosis, and numerical variation of chromosomes, where the diploid number ranges from 26 + X1 X2 Y to 26 + X1-20 Y. It is desirable to assess the nuclear DNA content of various cytotypes for a further detailed study of the C. lectularius genome. Detailed knowledge of the DNA content of this parasite could also clarify the origin of additional chromosomes. The average nuclear genome size C. lectularius with 2n = 26 + X1 X2 Y is 2C = 1.94 pg for males and 1.95 pg for females. There is a significant correlation between genome size and the number of chromosomes, but in some specimens with additional chromosomes, nuclear genome size decreases or remains average. Several species used as the internal reference standard were tested for further investigations of genome size in C. lectularius, and the plant Solanum pseudocaspicum turned out to be the most suitable. © 2019 International Society for Advancement of Cytometry.
Faculty of Science Department of Botany Charles University Praha Czech Republic
Faculty of Science Department of Zoology Charles University Praha Czech Republic
Institute of Entomology Biological Centre Czech Academy of Science České Budějovice Czech Republic
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