Novel influenza inhibitors designed to target PB1 interactions with host importin RanBP5
Language English Country Netherlands Media print-electronic
Document type Journal Article
PubMed
30742842
DOI
10.1016/j.antiviral.2019.02.003
PII: S0166-3542(18)30487-X
Knihovny.cz E-resources
- Keywords
- Antiviral, Nuclear localization, Polymerase inhibition, Virtual screening,
- MeSH
- beta Karyopherins metabolism MeSH
- Influenza, Human drug therapy MeSH
- Humans MeSH
- Drug Discovery MeSH
- Computer Simulation MeSH
- Virus Replication drug effects MeSH
- High-Throughput Screening Assays MeSH
- Molecular Docking Simulation MeSH
- Viral Proteins antagonists & inhibitors MeSH
- Influenza A virus drug effects enzymology MeSH
- Structure-Activity Relationship MeSH
- Check Tag
- Humans MeSH
- Publication type
- Journal Article MeSH
- Names of Substances
- beta Karyopherins MeSH
- influenza virus polymerase basic protein 1 MeSH Browser
- IPO5 protein, human MeSH Browser
- Viral Proteins MeSH
In search of novel targets for influenza inhibitors, a site on PB1 was selected for its high conservation and probable interaction with a host protein, RanBP5, that is key to nuclear import of PB1, where it complexes with PB2, PA, and NP to transcribe viral RNA. Docking with libraries of drug-like compounds led to a selection of five candidates that bound tightly and with a pose likely to inhibit protein binding. These were purchased and tested in vitro, found to be active, and then one was synthetically expanded to explore the structure-activity relationship. The top candidates had a carboxylic acid converted to an ester and electron-withdrawing substituents added to a phenyl group in the original structure. Resistance was slow to develop, but cytotoxicity was moderately high. Nuclear localization of PB1 and in vitro polymerase activity were both strongly inhibited.
Department of Chemistry and Biochemistry Brigham Young University USA
Department of Physiology and Developmental Biology Brigham Young University Provo UT 84602 USA
Institute of Organic Chemistry and Biochemistry of the CAS Prague Czech Republic
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