OBJECTIVES: The aim of this study was to analyse the DNA sequences of three teicoplanin-resistant Staphylococcus epidermidis isolates collected from patients not previously treated with glycopeptide antibiotics. METHODS: The minimum inhibitory concentrations (MICs) of 12 antibiotics, including teicoplanin and vancomycin, were determined by the broth microdilution method. Genomic DNA was isolated, was sequenced by HiSeqX paired-end sequencing and was assembled into draft genome sequences using MyPro pipeline. RESULTS: Analysis of the draft genome sequences demonstrated that the teicoplanin-resistant S. epidermidis isolates belonged to multilocus sequence typing (MLST) sequence types ST5 and ST87 and encoded multiple antimicrobial resistance genes, including the methicillin resistance gene mecA. CONCLUSIONS: This report highlights the risk of dissemination of S. epidermidis strains resistant to a wide range of clinically important antibiotics.

Clinical Microbiology and ATB Centre Institute of Medical Biochemistry and Laboratory Diagnostics General University Hospital Prague Czech Republic

Clinical Microbiology and ATB Centre Institute of Medical Biochemistry and Laboratory Diagnostics General University Hospital Prague Czech Republic; Department of Medical Microbiology Medical Faculty of Palacký University Olomouc Czech Republic

Laboratory for Biology of Secondary Metabolism Institute of Microbiology of the Academy of Sciences of the Czech Republic BIOCEV Průmyslová 595 252 50 Vestec Czech Republic

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