Herein, we present direct experimental evidence of pH oscillatory dynamics in the urea-urease enzymatic reaction conducted in a continuous reactor-membrane-reservoir system. Our results are consistent with earlier model predictions requiring differential transport of H+ and substrate. We report oscillations with periods in hundreds of seconds and the amplitude of ∼0.1 pH units.

Department of Chemical Engineering University of Chemistry and Technology Prague Technická 5 166 28 Praha 6 Czech Republic

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