Immunohistochemical analysis of retraction pocket pars tensa of tympanic membrane in children
Jazyk angličtina Země Irsko Médium print-electronic
Typ dokumentu časopisecké články
PubMed
30999159
DOI
10.1016/j.ijporl.2019.04.008
PII: S0165-5876(19)30170-3
Knihovny.cz E-zdroje
- Klíčová slova
- Chidren, Cholesteatoma, Immunohistochemistry, Pars tensa, Retraction pocket,
- MeSH
- antigen Ki-67 metabolismus MeSH
- antigeny CD31 metabolismus MeSH
- antigeny CD45 metabolismus MeSH
- biologické markery metabolismus MeSH
- cholesteatom středního ucha metabolismus MeSH
- dítě MeSH
- imunohistochemie MeSH
- lidé MeSH
- matrixová metaloproteinasa 9 metabolismus MeSH
- membrana tympani metabolismus MeSH
- prospektivní studie MeSH
- Check Tag
- dítě MeSH
- lidé MeSH
- Publikační typ
- časopisecké články MeSH
- Názvy látek
- antigen Ki-67 MeSH
- antigeny CD31 MeSH
- antigeny CD45 MeSH
- biologické markery MeSH
- matrixová metaloproteinasa 9 MeSH
- MMP9 protein, human MeSH Prohlížeč
- PTPRC protein, human MeSH Prohlížeč
AIMS: Immunohistochemical analysis of retraction pocket pars tensa of tympanic membrane in children. Identification of signs typical for cholesteatoma and support of retraction theory of cholesteatoma. STUDY DESIGN: a prospective study analysing 31 surgically removed retraction pockets. DEPARTMENT: University Hospital, Children's Medical Centre Methods: Retraction pockets processed by a standard process for immunohistochemical analysis. The observed findings were specified using antibodies CD45 LCA (leukocyte common antigen), CD31 (platelet endothelial cell adhesion molecule), D2-40 (marker of lymphatic endothelium), MMP9 (marker of degradation of connective tissue extracellular matrix) and Ki67 (cellular marker of proliferation). RESULTS: All observed parameters except for MMP9 had a significantly higher incidence in retraction pocket stage III compared to stage II according to Charachon. CONCLUSION: We described immunohistochemical signs of retraction pocket pars tensa of tympanic membrane in children resulting in cholesteatoma. All the observed signs occur in the structure of matrix and perimatrix of cholesteatoma. A significantly higher incidence of all observed parameters except from MMP9 was proved in retraction pocket stage III, unlike in stage II. This observation proves the fact that retraction pocket is a progressive disease and is a procholesteatoma stage.
Citace poskytuje Crossref.org
