Immunohistochemical analysis of retraction pocket pars tensa of tympanic membrane in children
Language English Country Ireland Media print-electronic
Document type Journal Article
PubMed
30999159
DOI
10.1016/j.ijporl.2019.04.008
PII: S0165-5876(19)30170-3
Knihovny.cz E-resources
- Keywords
- Chidren, Cholesteatoma, Immunohistochemistry, Pars tensa, Retraction pocket,
- MeSH
- Ki-67 Antigen metabolism MeSH
- Platelet Endothelial Cell Adhesion Molecule-1 metabolism MeSH
- Leukocyte Common Antigens metabolism MeSH
- Biomarkers metabolism MeSH
- Cholesteatoma, Middle Ear metabolism MeSH
- Child MeSH
- Immunohistochemistry MeSH
- Humans MeSH
- Matrix Metalloproteinase 9 metabolism MeSH
- Tympanic Membrane metabolism MeSH
- Prospective Studies MeSH
- Check Tag
- Child MeSH
- Humans MeSH
- Publication type
- Journal Article MeSH
- Names of Substances
- Ki-67 Antigen MeSH
- Platelet Endothelial Cell Adhesion Molecule-1 MeSH
- Leukocyte Common Antigens MeSH
- Biomarkers MeSH
- Matrix Metalloproteinase 9 MeSH
- MMP9 protein, human MeSH Browser
- PTPRC protein, human MeSH Browser
AIMS: Immunohistochemical analysis of retraction pocket pars tensa of tympanic membrane in children. Identification of signs typical for cholesteatoma and support of retraction theory of cholesteatoma. STUDY DESIGN: a prospective study analysing 31 surgically removed retraction pockets. DEPARTMENT: University Hospital, Children's Medical Centre Methods: Retraction pockets processed by a standard process for immunohistochemical analysis. The observed findings were specified using antibodies CD45 LCA (leukocyte common antigen), CD31 (platelet endothelial cell adhesion molecule), D2-40 (marker of lymphatic endothelium), MMP9 (marker of degradation of connective tissue extracellular matrix) and Ki67 (cellular marker of proliferation). RESULTS: All observed parameters except for MMP9 had a significantly higher incidence in retraction pocket stage III compared to stage II according to Charachon. CONCLUSION: We described immunohistochemical signs of retraction pocket pars tensa of tympanic membrane in children resulting in cholesteatoma. All the observed signs occur in the structure of matrix and perimatrix of cholesteatoma. A significantly higher incidence of all observed parameters except from MMP9 was proved in retraction pocket stage III, unlike in stage II. This observation proves the fact that retraction pocket is a progressive disease and is a procholesteatoma stage.
References provided by Crossref.org
Cartilage tympanoplasty for retraction pocket of the tympanic membrane in children
Pathogenesis of Retraction Pocket of the Tympanic Membrane-A Narrative Review
