Genetic diversity and multiple antibiotic resistance index study of bacterial pathogen, Klebsiella pneumoniae strains isolated from diseased Indian major carps
Jazyk angličtina Země Spojené státy americké Médium print-electronic
Typ dokumentu časopisecké články
Grantová podpora
G/Nat. Surveillance/2013 dated 16.08.2013
NFDB
PubMed
31020632
DOI
10.1007/s12223-019-00701-7
PII: 10.1007/s12223-019-00701-7
Knihovny.cz E-zdroje
- Klíčová slova
- Antibiotic resistance index, Carp fish, Genetic diversity, India, Klebsiella pneumoniae,
- MeSH
- antibakteriální látky farmakologie MeSH
- bakteriální geny genetika MeSH
- DNA bakterií genetika MeSH
- fylogeneze MeSH
- genetická variace MeSH
- hemolýza MeSH
- infekce bakteriemi rodu Klebsiella epidemiologie mikrobiologie veterinární MeSH
- kapři mikrobiologie MeSH
- Klebsiella pneumoniae klasifikace účinky léků genetika izolace a purifikace MeSH
- mezerníky ribozomální DNA genetika MeSH
- mikrobiální testy citlivosti MeSH
- mnohočetná bakteriální léková rezistence * MeSH
- nemoci ryb mikrobiologie MeSH
- prevalence MeSH
- ribotypizace MeSH
- RNA ribozomální 16S genetika MeSH
- sekvenční analýza DNA MeSH
- vodní hospodářství MeSH
- zvířata MeSH
- Check Tag
- zvířata MeSH
- Publikační typ
- časopisecké články MeSH
- Geografické názvy
- Indie epidemiologie MeSH
- Názvy látek
- antibakteriální látky MeSH
- DNA bakterií MeSH
- mezerníky ribozomální DNA MeSH
- RNA ribozomální 16S MeSH
Intensive fish farming systems have led to increase in disease incidence, due to higher stocking density, high organic matter levels, and poor quality of the aquatic environment. Diseased fish samples showing hemorrhages and reddish lesions were collected from different freshwater fish farms located at three different districts of West Bengal, India (Burdwan, North 24 Parganas, and Nadia). The present study was conducted to evaluate the genetic diversity of ten different Klebsiella pneumoniae strains isolated from different infected freshwater fish samples based on 16S rRNA gene sequence analysis. Primarily, Klebsiella-specific media was used for the isolation and characterization of Klebsiella pneumoniae. Further, through a biochemical test, all the strains were confirmed as K. pneumoniae. PCR analysis of 16S-23S internal transcribed spacer (PCR ribotyping) was carried out to study the species variation within different Klebsiella pneumoniae isolates. For all the isolates, a conserved PCR ribotype pattern was observed while differing from other bacterial species. Phylogenetic study showed the high degree of homology with diverse source of other strains. The multiple antibiotic resistance (MAR) values of the present study for the isolates were found to be 0.468. MAR value above 0.2 indicates that the source of isolation was highly contaminated with antibiotics. Based on the 16S rRNA gene sequence analysis, the present study revealed the genetic diversity of Klebsiella pneumoniae isolated from the different diseased fish farms of West Bengal. All the strains were found to be hypermucoviscous and multidrug-resistant, thus making it pathogenic towards the host organisms. Further, the study revealed a high prevalence of K. pneumoniae in aquaculture farms, representing a risk towards successful aquaculture.
Department of Zoology Vidyasagar University Medinipur West Bengal 721102 India
ICAR Central Inland Fisheries Research Institute Barrackpore Kolkata West Bengal 700120 India
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