Expression of selected mitochondrial genes during in vitro maturation of bovine oocytes related to their meiotic competence
Jazyk angličtina Země Spojené státy americké Médium print-electronic
Typ dokumentu časopisecké články
PubMed
31078068
DOI
10.1016/j.theriogenology.2019.05.001
PII: S0093-691X(19)30120-7
Knihovny.cz E-zdroje
- Klíčová slova
- Bovine, Gene expression, Maturation, Mitochondria, Oocyte,
- MeSH
- IVM techniky veterinární MeSH
- mitochondriální DNA biosyntéza MeSH
- mitochondriální geny * MeSH
- mitochondriální proteiny genetika metabolismus MeSH
- oocyty metabolismus MeSH
- skot fyziologie MeSH
- zvířata MeSH
- Check Tag
- skot fyziologie MeSH
- ženské pohlaví MeSH
- zvířata MeSH
- Publikační typ
- časopisecké články MeSH
- Názvy látek
- mitochondriální DNA MeSH
- mitochondriální proteiny MeSH
The main goal of this study was to characterize the expression patterns of genes which play a role in mitochondrial DNA biogenesis and metabolism during the maturation of bovine oocytes with different meiotic competence and health. Meiotically more and less competent oocytes were obtained separately either from medium (MF) or small (SF) follicles and categorized according to oocyte morphology into healthy and light-atretic. The four oocyte categories were matured and collected after 0, 3, 7, 16 and 24 h of maturation. Either total RNA or poly(A) RNA were extracted from oocytes and the expression of selected mitochondrial translational factors (TFAM, TFB1M, and TFB2M), MATER, and Luciferase as external standard was assessed using a real-time RT-PCR. The level of TFAM, TFB1M and MATER poly(A) RNA transcripts significantly decreased during maturation in both healthy and light-atretic MF and SF oocytes. On the other hand, the level of TFB2M poly(A) increased during maturation in healthy and light-atretic SF oocytes, in contrast to MF oocytes. The abundance of TFAM total RNA was significantly higher after maturation than that before maturation in all oocyte categories. However, no differences in TFB1M and TFB2M total RNA were found in any oocyte categories. It can be concluded that the gene expression patterns differ in maturing bovine oocytes in dependence on their meiotic competence and health. The TFAM and TFB1M poly(A) RNAs are actively deadenylated at different meiotic stages but TFB2M poly(A) RNA remains elevated in light-atretic less competent oocytes until the completion of meiosis.
Institute of Animal Physiology and Genetics of the Czech Academy of Sciences Libechov Czech Republic
Citace poskytuje Crossref.org
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