The method of long-term catheterization of the vena jugularis in pigs
Jazyk angličtina Země Spojené státy americké Médium print-electronic
Typ dokumentu časopisecké články
PubMed
31100450
DOI
10.1016/j.vascn.2019.106584
PII: S1056-8719(19)30064-4
Knihovny.cz E-zdroje
- Klíčová slova
- Jugular vein, Permanent intravenous catheterization, Pig,
- MeSH
- katetrizace metody MeSH
- odběr vzorku krve metody MeSH
- prasata MeSH
- taurin aplikace a dávkování analogy a deriváty MeSH
- thiadiaziny aplikace a dávkování MeSH
- venae jugulares chirurgie MeSH
- zvířata MeSH
- Check Tag
- mužské pohlaví MeSH
- ženské pohlaví MeSH
- zvířata MeSH
- Publikační typ
- časopisecké články MeSH
- Názvy látek
- taurin MeSH
- taurolidine MeSH Prohlížeč
- thiadiaziny MeSH
INTRODUCTION: The pig is one of the most valuable in vivo models in biomedical research, however with only a few well-accessible veins suitable for venipuncture. Moreover, most of the known methods of blood collection are suitable only for a limited time period. The aim of the study was to verify an improved method of long-term catheterization of the jugular vein in pigs. METHODS: A 420 mm polyurethane catheter 16G tube was surgically inserted using the Seldinger technique. The part of the tube that was not inserted into the vein was threaded through a subcutaneously introduced trocar into the occipital area, where it was well accessible and well protected from damage. The catheters were flushed with sterile 0.9% saline solution and locked with 4% citrate between frequent blood samplings, or with 30% citrate at intervals of 1-2-days. Once a week, the catheters were locked with 4% citrate containing taurolidine for 24 h in order to prevent infection. The method was verified in 14 pigs. RESULTS: The catheters were fully functional for up to 11 weeks and no infection or thrombus was observed. DISCUSSION: This method of catheterization and catheter care allows the realization of long-term experiments with comfortable and stress-free blood sampling.
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