Visualizing and Quantifying In Vivo Cortical Cytoskeleton Structure and Dynamics
Language English Country United States Media print
Document type Journal Article, Research Support, Non-U.S. Gov't
- Keywords
- Actin, CLSM, Fluorescent proteins, Image analysis, ImageJ, Microtubules, SDCM, VAEM,
- MeSH
- Arabidopsis ultrastructure MeSH
- Cytoskeleton ultrastructure MeSH
- Microscopy, Fluorescence methods MeSH
- Microscopy, Confocal methods MeSH
- Microtubules ultrastructure MeSH
- Image Processing, Computer-Assisted methods MeSH
- Plant Cells ultrastructure MeSH
- Publication type
- Journal Article MeSH
- Research Support, Non-U.S. Gov't MeSH
The cortical microtubule and actin meshworks play a central role in the shaping of plant cells. Transgenic plants expressing fluorescent protein markers specifically tagging the two main cytoskeletal systems are available, allowing noninvasive in vivo studies. Advanced microscopy techniques, in particular confocal laser scanning microscopy (CLSM), spinning disk confocal microscopy (SDCM), and variable angle epifluorescence microscopy (VAEM), can be nowadays used for imaging the cortical cytoskeleton of living cells with unprecedented spatial and temporal resolution. With the aid of free computing tools based on the publicly available ImageJ software package, quantitative information can be extracted from microscopic images and video sequences, providing insight into both architecture and dynamics of the cortical cytoskeleton.
Coordinación de Innovación Regional C 1 Turipaná Montería Córdoba Colombia
Department of Experimental Plant Biology Faculty of Science Charles University Prague Czech Republic
Institute of Experimental Botany of the Czech Academy of Sciences Prague Czech Republic
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