The Photoconvertible Fluorescent Protein Dendra2 Tag as a Tool to Investigate Intracellular Protein Dynamics
Methods in molecular biology (Clifton, N.J.).
2019 ;
1992 () :
201-214.
Language English Country United States Media print
Document type Journal Article, Research Support, Non-U.S. Gov't
Persistent link
https://www.medvik.cz/link/pmid31148040
- Keywords
- Arabidopsis SYT1, Dendra2, Photoactivated localization microscopy, Photoconvertible fluorescence proteins,
- MeSH
- Arabidopsis chemistry cytology ultrastructure MeSH
- Fluorescent Dyes analysis MeSH
- Intracellular Membranes chemistry ultrastructure MeSH
- Microscopy, Confocal methods MeSH
- Plant Roots chemistry ultrastructure MeSH
- Luminescent Proteins analysis MeSH
- Arabidopsis Proteins analysis MeSH
- Light MeSH
- Synaptotagmin I analysis MeSH
- Publication type
- Journal Article MeSH
- Research Support, Non-U.S. Gov't MeSH
- Names of Substances
- Fluorescent Dyes MeSH
- Luminescent Proteins MeSH
- Arabidopsis Proteins MeSH
- Synaptotagmin I MeSH
- SYT1 protein, Arabidopsis MeSH Browser
Fluorescence proteins changing spectral properties after exposure to light with a specific wavelength have recently become outstanding aids in the study of intracellular protein dynamics. Herein we show using Arabidopsis SYNAPTOTAGMIN 1 as a model protein that the Dendra2 green to red photoconvertible protein tag in combination with confocal scanning laser microscopy is a useful tool to study membrane protein intracellular dynamics.
References provided by Crossref.org
International journal of molecular sciences.
2022 Feb 03 ;
23 (3) :
.
[epub] 20220203
