Pseudogemmobacter bohemicus gen. nov., sp. nov., a novel taxon from the Rhodobacteraceae family isolated from heavy-metal-contaminated sludge
Language English Country England, Great Britain Media print-electronic
Document type Journal Article
- Keywords
- 16S rRNA gene analysis, Pseudogemmobacter bohemicus, Rhodobacteraceae, chemotaxonomic characterization, heavy metals, percentage of conserved proteins,
- MeSH
- DNA, Bacterial genetics MeSH
- Phospholipids chemistry MeSH
- Phylogeny * MeSH
- Fatty Acids chemistry MeSH
- Sewage microbiology MeSH
- Rhodobacteraceae classification isolation & purification MeSH
- RNA, Ribosomal, 16S genetics MeSH
- Sequence Analysis, DNA MeSH
- Bacterial Typing Techniques MeSH
- Metals, Heavy * MeSH
- Ubiquinone analogs & derivatives chemistry MeSH
- Base Composition MeSH
- Publication type
- Journal Article MeSH
- Geographicals
- Czech Republic MeSH
- Names of Substances
- coenzyme Q10 MeSH Browser
- DNA, Bacterial MeSH
- Phospholipids MeSH
- Fatty Acids MeSH
- Sewage MeSH
- RNA, Ribosomal, 16S MeSH
- Metals, Heavy * MeSH
- Ubiquinone MeSH
The creamy white to beige, aerobic, non-motile, ovoid to rod-shaped, Gram-stain-negative strain, Cd-10T, was isolated from heavy-metal-contaminated sludge from a decantation basin of a heavy metal processing factory based on its ability to tolerate CdCl2 in the cultivation medium. In the reconstruction of its phylogeny based on 16S rRNA gene sequences, strain Cd-10T clustered with species of the genera Gemmobacter, Xinfangfangia, Tabrizicola and Rhodobacter within the family Rhodobacteraceae. Its 16S rRNA gene sequence exhibited 96.32 % pairwise similarity to the type strain of Xinfangfangia soli, 95.3 % to that of Gemmobacter intermedius, followed by Tabrizicola fusiformis (95.10 %), Rhodobacter sediminis (94.88 %), Gemmobacter nectariphilus and Rhodobacter capsulatus (both 94.81 %). The major respiratory quinone was Q-10 accompanied by Q-9, the fatty acid profile consisted predominantly of C18 : 1ω7c, C18 : 0, C16 : 0 and C16 : 1ω7c, the major polar lipids were phosphatidylglycerol, phosphatidylmethylethanolamine, phosphatidylcholine and diphosphatidylglycerol. An analysis of the percentage of conserved proteins deduced from draft or complete genomic sequences of strain Cd-10T and representatives of its closest relatives suggested that strain Cd-10T is a member of a novel genus within the Rhodobacteraceae family for which we propose the name Pseudogemmobacter. Strain Cd-10T (=DSM 103618T=NCCB 100645T) is the type strain of Pseudogemmobacter bohemicus gen. nov., sp. nov., the type species of the genus Pseudogemmobacter gen. nov.
Institute for Environmental Studies Faculty of Science Charles University Prague Czech Republic
Institute of Microbiology Czech Academy of Sciences v v i Prague Czech Republic
Institute of Molecular Genetics Czech Academy of Sciences v v i Prague Czech Republic
References provided by Crossref.org
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