Genistein and Selected Phytoestrogen-Containing Extracts Differently Modulate Antioxidant Properties and Cell Differentiation: an in Vitro Study in NIH-3T3, HaCaT and MCF-7 Cells
Jazyk angličtina Země Česko Médium print
Typ dokumentu časopisecké články
PubMed
31171079
DOI
10.14712/fb2019065010024
PII: file/5888/fb2019a0003.pdf
Knihovny.cz E-zdroje
- MeSH
- antioxidancia metabolismus MeSH
- buněčná diferenciace účinky léků MeSH
- buňky NIH 3T3 MeSH
- fytoestrogeny chemie MeSH
- genistein chemie MeSH
- keratin-8 metabolismus MeSH
- lidé MeSH
- MFC-7 buňky MeSH
- myši MeSH
- proliferace buněk účinky léků MeSH
- rostlinné extrakty chemie farmakologie MeSH
- zvířata MeSH
- Check Tag
- lidé MeSH
- myši MeSH
- zvířata MeSH
- Publikační typ
- časopisecké články MeSH
- Názvy látek
- antioxidancia MeSH
- fytoestrogeny MeSH
- genistein MeSH
- keratin-8 MeSH
- rostlinné extrakty MeSH
During the last decades, plant extracts containing phytoestrogens have increasingly been used as an alternative to oestradiol hormone replacement therapy. The aim of the present study was to compare the effects of genistein with those of different phytoestrogen-containing plant extracts (from red clover flowers and soybeans) on the proliferation and differentiation of NIH-3T3, HaCaT and MCF-7 cells. Our results showed poor correlations between direct anti/pro-oxidant effects and cytotoxicity of the tested samples. In contrast, genistein showed a direct correlation between significant pro-oxidative effects at cytotoxic concentrations and almost no pro-oxidative effects at non-cytotoxic concentrations. Moreover, the tested red clover extract and genistein induced keratin-8 (luminal and prognostic marker in breast cancer) expression only in MCF-7 cells, but this effect was not seen following treatment with the soybean extract. From this point of view, the effect of consumption of phytoestrogens in oestrogen-positive breast cancer remains to be elucidated. In conclusion, our study demonstrates that various phytoestrogen- containing plant extracts and genistein are able to specifically modulate antioxidant properties and differentiation of studied cells.
BIOCEV 1st Faculty of Medicine Charles University Vestec Czech Republic
Burn Centre Prague 3rd Faculty of Medicine Charles University Prague Czech Republic
Institute of Anatomy 1st Faculty of Medicine Charles University Prague Czech Republic
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