Hydrogels belong to the group of materials with growing interest on the market of polymers. In this article, hydrogels based on Beetosan were obtained using ultraviolet (UV) radiation. Main component of hydrogel matrix-Beetosan-is chitosan obtained from naturally died honeybees. Such hydrogels were modified with active substances, that is, caffeine, bee pollen, Salvia officinalis (sage), and Aloe vera juice. Next, the analysis of cytotoxicity of hydrogels in relation to murine fibroblasts by 3-[4,5-dimethylthiazol-2-yl]-2,5-diphenyltetrazolium bromide and neutral red uptake assays were conducted. Furthermore, surface morphology, tensile strength, geometry, and roughness of hydrogels were characterized. Hydrogels did not show cytotoxicity to recommended L929 murine fibroblasts. These polymers did not affect adversely the growth and viability of these cells. Moreover, Beetosan hydrogels were characterized by flexibility as well as by diversified surface morphology that could indicate their high absorbency. Therefore these materials may be considered as useful for biomedical purposes with special emphasis on application as modern wound dressings that not only absorb wound exudate but also contain natural substances with therapeutic properties that is beneficial from the point of view of wound healing process.

Faculty of Biology and Environmental Protection Department of Immunology and Infectious Biology University of Lodz Łódź Poland

Faculty of Biology and Environmental Protection Department of Immunoparasitology University of Lodz Łódź Poland

Faculty of Chemical Engineering and Technology Department of Chemistry and Technology of Polymers Cracow University of Technology Krakow Poland

Faculty of Chemical Engineering and Technology Institute of Inorganic Chemistry and Technology Cracow University of Technology Krakow Poland

Faculty of Natural Sciences Department of Analytical Chemistry Comenius University Bratislava Slovakia

Faculty of Science Regional Centre of Advanced Technologies and Materials Palacky University Olomouc Czech Republic

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