GFP transgenic animals in biomedical research: a review of potential disadvantages
Language English Country Czech Republic Media print-electronic
Document type Journal Article, Review
PubMed
31342754
DOI
10.33549/physiolres.934227
PII: 934227
Knihovny.cz E-resources
- MeSH
- Biomedical Research methods trends MeSH
- Zebrafish MeSH
- Animals, Genetically Modified genetics MeSH
- Rabbits MeSH
- Mice, Transgenic MeSH
- Mice MeSH
- Gene Expression Regulation, Developmental MeSH
- Green Fluorescent Proteins biosynthesis genetics MeSH
- Animals MeSH
- Check Tag
- Rabbits MeSH
- Mice MeSH
- Animals MeSH
- Publication type
- Journal Article MeSH
- Review MeSH
- Names of Substances
- Green Fluorescent Proteins MeSH
Green Fluorescent protein (GFP) transgenic animals are accepted tools for studying various physiological processes, including organ development and cell migration. However, several in vivo studies claimed that GFP may impair transgenic animals' health. Glomerulosclerosis was observed in transgenic mice and rabbits with ubiquitous reporter protein expression. Heart-specific GFP expression evoked dilated cardiomyopathy and altered cardiac function in transgenic mouse and zebrafish lines, respectively. Moreover, growth retardation and increased axon swelling were observed in GFP and yellow fluorescent protein (YFP) transgenic mice, respectively. This review will focus on the potential drawbacks of the applications of GFP transgenic animals in biomedical research.
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