2Hz-electroacupuncture attenuates heroin-seeking behaviors via adjusts CB1-Rs and CB2-Rs expression in relapse-relevant brain regions of heroin self-administration rats
Language English Country Czech Republic Media print-electronic
Document type Journal Article
PubMed
31424247
DOI
10.33549/physiolres.934106
PII: 934106
Knihovny.cz E-resources
- MeSH
- Self Administration MeSH
- Drug-Seeking Behavior drug effects MeSH
- Behavior, Animal drug effects MeSH
- Electroacupuncture * MeSH
- Extinction, Psychological drug effects MeSH
- Heroin administration & dosage MeSH
- Locomotion drug effects MeSH
- Disease Models, Animal MeSH
- Brain drug effects metabolism physiopathology MeSH
- Narcotic Antagonists administration & dosage MeSH
- Rats, Sprague-Dawley MeSH
- Receptor, Cannabinoid, CB1 metabolism MeSH
- Receptor, Cannabinoid, CB2 metabolism MeSH
- Recurrence MeSH
- Signal Transduction MeSH
- Heroin Dependence metabolism physiopathology psychology therapy MeSH
- Animals MeSH
- Check Tag
- Male MeSH
- Animals MeSH
- Publication type
- Journal Article MeSH
- Names of Substances
- Cnr1 protein, rat MeSH Browser
- Cnr2 protein, rat MeSH Browser
- Heroin MeSH
- Narcotic Antagonists MeSH
- Receptor, Cannabinoid, CB1 MeSH
- Receptor, Cannabinoid, CB2 MeSH
Opiate addiction has a high rate of relapse. The accumulating evidence shows that electroacupuncture (EA) may be effective for the treatment of opiate relapse. However, the change of expression of CB1-Rs and CB2-Rs involve in 2Hz EA anti-relapse pathway is still unclear. To explore the changes of expression of CB1-Rs and CB2-Rs, heroin self-administration (SA) model rats were adopted and treated using 2Hz EA. The expressions of CB1-Rs and CB2-Rs were observed using immunohistochemistry method. The results showed that, compared with the control group, active pokes in the heroin-addicted group increased, while the active pokes decreased significantly in 2Hz EA group compared with heroin-addicted group. Correspondingly, the expression of CB1-Rs in prefrontal cortex (PFC), hippocampus (Hip), nucleus accumbens (NAc) and ventral tegmental area (VTA) all increased significantly while the expression of CB2-Rs in those relapse-relevant brain regions decreased obviously in heroin-addicted group when compared with the control group. In addition, the expression of CB1-Rs obviously decreased in the 2Hz EA group while the expression of CB2-Rs in those relapse-relevant brain regions increased significantly when compared with the heroin-addicted group. It indicated that 2Hz EA could attenuate the heroin-evoked seeking behaviors effectively. The anti-relapse effects of 2Hz EA might be related to the decrease of CB1-Rs and increase of CB2-Rs expression in relapse-relevant brain regions of heroin SA rats.
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