A Molecular Framework for the Control of Adventitious Rooting by TIR1/AFB2-Aux/IAA-Dependent Auxin Signaling in Arabidopsis
Language English Country England, Great Britain Media print-electronic
Document type Journal Article, Research Support, Non-U.S. Gov't
PubMed
31520787
DOI
10.1016/j.molp.2019.09.001
PII: S1674-2052(19)30290-4
Knihovny.cz E-resources
- Keywords
- Arabidopsis, AuxIAA, TIR1/AFB, adventitious roots, jasmonate,
- MeSH
- Arabidopsis cytology genetics growth & development metabolism MeSH
- F-Box Proteins metabolism MeSH
- Hypocotyl metabolism MeSH
- Plant Roots growth & development MeSH
- Indoleacetic Acids metabolism MeSH
- Arabidopsis Proteins metabolism MeSH
- Receptors, Cell Surface metabolism MeSH
- Gene Expression Regulation, Plant MeSH
- Signal Transduction * MeSH
- Protein Stability MeSH
- Publication type
- Journal Article MeSH
- Research Support, Non-U.S. Gov't MeSH
- Names of Substances
- AFB2 protein, Arabidopsis MeSH Browser
- F-Box Proteins MeSH
- indoleacetic acid MeSH Browser
- Indoleacetic Acids MeSH
- Arabidopsis Proteins MeSH
- Receptors, Cell Surface MeSH
- TIR1 protein, Arabidopsis MeSH Browser
In Arabidopsis thaliana, canonical auxin-dependent gene regulation is mediated by 23 transcription factors from the AUXIN RESPONSE FACTOR (ARF) family that interact with auxin/indole acetic acid repressors (Aux/IAAs), which themselves form co-receptor complexes with one of six TRANSPORT INHIBITOR1/AUXIN-SIGNALLING F-BOX (TIR1/AFB) proteins. Different combinations of co-receptors drive specific sensing outputs, allowing auxin to control a myriad of processes. ARF6 and ARF8 are positive regulators of adventitious root initiation upstream of jasmonate, but the exact auxin co-receptor complexes controlling the transcriptional activity of these proteins has remained unknown. Here, using loss-of-function mutants we show that three Aux/IAA genes, IAA6, IAA9, and IAA17, act additively in the control of adventitious root (AR) initiation. These three IAA proteins interact with ARF6 and/or ARF8 and likely repress their activity in AR development. We show that TIR1 and AFB2 are positive regulators of AR formation and TIR1 plays a dual role in the control of jasmonic acid (JA) biosynthesis and conjugation, as several JA biosynthesis genes are up-regulated in the tir1-1 mutant. These results lead us to propose that in the presence of auxin, TIR1 and AFB2 form specific sensing complexes with IAA6, IAA9, and/or IAA17 to modulate JA homeostasis and control AR initiation.
Institut Jean Pierre Bourgin INRA AgroParisTech CNRS Université Paris Saclay 78000 Versailles France
Umeå Plant Science Centre Department of Plant Physiology Umeå University 90736 Umeå Sweden
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