Circulating Endometrial Cells in Women With Spontaneous Pneumothorax
Jazyk angličtina Země Spojené státy americké Médium print-electronic
Typ dokumentu časopisecké články, práce podpořená grantem
PubMed
31542450
DOI
10.1016/j.chest.2019.09.008
PII: S0012-3692(19)33873-5
Knihovny.cz E-zdroje
- Klíčová slova
- MetaCell, biomarker, catamenial pneumothorax, circulating endometrial cells, culturing, endometriosis, gene expression profiling, in vitro, liquid biopsy,
- MeSH
- antigen CA-125 genetika MeSH
- dospělí MeSH
- endometrióza krev genetika MeSH
- endometrium cytologie MeSH
- keratin-18 genetika MeSH
- lidé středního věku MeSH
- lidé MeSH
- membránové proteiny genetika MeSH
- mladý dospělý MeSH
- mucin 1 genetika MeSH
- nemoci pleury krev genetika MeSH
- pneumotorax krev diagnóza genetika MeSH
- receptor erbB-2 genetika MeSH
- studie případů a kontrol MeSH
- tekutá biopsie MeSH
- transkriptom MeSH
- vimentin genetika MeSH
- Check Tag
- dospělí MeSH
- lidé středního věku MeSH
- lidé MeSH
- mladý dospělý MeSH
- ženské pohlaví MeSH
- Publikační typ
- časopisecké články MeSH
- práce podpořená grantem MeSH
- Názvy látek
- antigen CA-125 MeSH
- ERBB2 protein, human MeSH Prohlížeč
- keratin-18 MeSH
- KRT18 protein, human MeSH Prohlížeč
- membránové proteiny MeSH
- MUC1 protein, human MeSH Prohlížeč
- MUC16 protein, human MeSH Prohlížeč
- mucin 1 MeSH
- receptor erbB-2 MeSH
- VIM protein, human MeSH Prohlížeč
- vimentin MeSH
BACKGROUND: The occurrence of catamenial pneumothorax (CP) is rare, and the awareness of this diagnosis among physicians is insufficient. CP is highly correlated with pelvic endometriosis and remains the most common form of thoracic endometriosis syndrome. Circulating endometrial cells (CECs) have been previously detected in patients with pelvic endometriosis. Could CECs bring new insights into pneumothorax management? METHODS: This study aims to describe the occurrence and molecular characteristics of CECs in women with spontaneous pneumothorax (SP) (N = 20) with high suspicion of its catamenial character. CECs were enriched from peripheral blood by size-based separation (MetaCell). In addition to cytomorphology, gene expression profiling of captured cells was performed for 24 endometriosis-associated genes. RESULTS: CECs were present in all 20 patients with SP. Enriched CECs exhibited four character features: epithelial, stem cell-like, stroma-like, and glandular. However, not all of them were present in every sampling. Gene expression profiling revealed two distinct phenotypes of CECs in SP and/or CP: one of them refers to the diaphragm openings syndrome and the other to endometrial tissue pleural implantations. Comparisons of the gene expression profiles of CECs in pneumothorax (CECs-SP group) with CECs in pelvic endometriosis (CECs-non-SP group) have revealed significantly higher expression of HER2 in the CECs-SP group compared with the CECs-non-SP group. CONCLUSIONS: This proof-of-concept study demonstrates successful isolation and characterization of CECs in patients with SP. Identification of CECs in SP could alert endometriosis involvement and help early referral to gynecologic consultation for further examination and treatment.
Department of Thoracic Surgery Krajska zdravotni a s Hospital Usti nad Labem Czech Republic
Department of Thoracic Surgery Lower Silesian Oncology Centre Wroclaw Poland
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