Circulating Endometrial Cells in Women With Spontaneous Pneumothorax
Language English Country United States Media print-electronic
Document type Journal Article, Research Support, Non-U.S. Gov't
PubMed
31542450
DOI
10.1016/j.chest.2019.09.008
PII: S0012-3692(19)33873-5
Knihovny.cz E-resources
- Keywords
- MetaCell, biomarker, catamenial pneumothorax, circulating endometrial cells, culturing, endometriosis, gene expression profiling, in vitro, liquid biopsy,
- MeSH
- CA-125 Antigen genetics MeSH
- Adult MeSH
- Endometriosis blood genetics MeSH
- Endometrium cytology MeSH
- Keratin-18 genetics MeSH
- Middle Aged MeSH
- Humans MeSH
- Membrane Proteins genetics MeSH
- Young Adult MeSH
- Mucin-1 genetics MeSH
- Pleural Diseases blood genetics MeSH
- Pneumothorax blood diagnosis genetics MeSH
- Receptor, ErbB-2 genetics MeSH
- Case-Control Studies MeSH
- Liquid Biopsy MeSH
- Transcriptome MeSH
- Vimentin genetics MeSH
- Check Tag
- Adult MeSH
- Middle Aged MeSH
- Humans MeSH
- Young Adult MeSH
- Female MeSH
- Publication type
- Journal Article MeSH
- Research Support, Non-U.S. Gov't MeSH
- Names of Substances
- CA-125 Antigen MeSH
- ERBB2 protein, human MeSH Browser
- Keratin-18 MeSH
- KRT18 protein, human MeSH Browser
- Membrane Proteins MeSH
- MUC1 protein, human MeSH Browser
- MUC16 protein, human MeSH Browser
- Mucin-1 MeSH
- Receptor, ErbB-2 MeSH
- VIM protein, human MeSH Browser
- Vimentin MeSH
BACKGROUND: The occurrence of catamenial pneumothorax (CP) is rare, and the awareness of this diagnosis among physicians is insufficient. CP is highly correlated with pelvic endometriosis and remains the most common form of thoracic endometriosis syndrome. Circulating endometrial cells (CECs) have been previously detected in patients with pelvic endometriosis. Could CECs bring new insights into pneumothorax management? METHODS: This study aims to describe the occurrence and molecular characteristics of CECs in women with spontaneous pneumothorax (SP) (N = 20) with high suspicion of its catamenial character. CECs were enriched from peripheral blood by size-based separation (MetaCell). In addition to cytomorphology, gene expression profiling of captured cells was performed for 24 endometriosis-associated genes. RESULTS: CECs were present in all 20 patients with SP. Enriched CECs exhibited four character features: epithelial, stem cell-like, stroma-like, and glandular. However, not all of them were present in every sampling. Gene expression profiling revealed two distinct phenotypes of CECs in SP and/or CP: one of them refers to the diaphragm openings syndrome and the other to endometrial tissue pleural implantations. Comparisons of the gene expression profiles of CECs in pneumothorax (CECs-SP group) with CECs in pelvic endometriosis (CECs-non-SP group) have revealed significantly higher expression of HER2 in the CECs-SP group compared with the CECs-non-SP group. CONCLUSIONS: This proof-of-concept study demonstrates successful isolation and characterization of CECs in patients with SP. Identification of CECs in SP could alert endometriosis involvement and help early referral to gynecologic consultation for further examination and treatment.
Department of Thoracic Surgery Krajska zdravotni a s Hospital Usti nad Labem Czech Republic
Department of Thoracic Surgery Lower Silesian Oncology Centre Wroclaw Poland
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