Circulating endometrial cells (CECs) have emerged as a new biomarker of advanced disease in women with endometriosis. The identification of several subtypes of CECs (e.g., stem cell-like, epithelial, glandular, stromal) has opened the way for characterization of endometriosis-associated CECs. This study focused on the isolation and characterization of CECs and disseminated endometrial cells (DECs) in patients with spontaneous pneumothorax (SP). The primary objective was to differentiate between cancer and non-cancer cells in patients with no previous cancer diagnosis. The MetaCell® size-based separation protocol was used to enrich CECs/DECs. Evaluation of the captured cells by 3D microscopy was performed using a NANOLIVETM microscope using a holographic approach. Based on gene expression analysis (GEA), we can conclude that mitochondria are much more active in primary tumors compared to endometriosis tissue (e.g. MT-ND1, MT-ATP6 genes). The culture of DECs is made of stromal, stem and immune cells. In vitro culture of DECs is characterized by an increase in the epithelial marker KRT18. Similarly, NFE2L2, a proerythroid factor, is also elevated. Further, a significant decrease in the amount of stem and immune cells was observed in the cell culture of DECs. The data presented here show how morphologically plastic the changes in the mitochondrial network can be and how cells can reflect them at the level of gene expression. The markers identified could help in the accompanying diagnostic process of the spontaneous pneumothorax in women of reproductive age.

• MeSH
• Adult MeSH
• Endometriosis * pathology   diagnosis   genetics   MeSH
• Endometrium pathology   metabolism   MeSH
• Humans MeSH
• Mitochondria * metabolism   pathology   MeSH
• Pneumothorax * pathology   diagnosis   MeSH
• Gene Expression Profiling methods   MeSH
• Check Tag
• Adult MeSH
• Humans MeSH
• Female MeSH
• Publication type
• Journal Article MeSH

• MeSH
• Central Venous Catheters classification   MeSH
• Humans MeSH
• Infant, Newborn MeSH
• Pleural Effusion diagnosis   etiology   therapy   MeSH
• Pneumothorax diagnosis   classification   therapy   MeSH
• Drainage, Postural * classification   methods   MeSH
• Radiography, Thoracic methods   MeSH
• Check Tag
• Humans MeSH
• Infant, Newborn MeSH
• Publication type
• Review MeSH

• MeSH
• Contraindications, Procedure MeSH
• Humans MeSH
• Myocardium MeSH
• Infant, Newborn MeSH
• Pericardial Effusion diagnosis   classification   therapy   MeSH
• Pericardiocentesis * classification   methods   adverse effects   MeSH
• Pneumothorax diagnosis   etiology   MeSH
• Cardiac Tamponade diagnosis   etiology   complications   MeSH
• Check Tag
• Humans MeSH
• Infant, Newborn MeSH
• Publication type
• Review MeSH

• MeSH
• Diagnostic Imaging methods   MeSH
• Diagnosis, Differential MeSH
• Drainage methods   MeSH
• Humans MeSH
• Oxygen Inhalation Therapy MeSH
• Pneumothorax * diagnosis   classification   therapy   MeSH
• Recurrence MeSH
• Check Tag
• Humans MeSH
• Publication type
• Review MeSH

Amyloidosis is a rare metabolic disorder primarily brought on by misfolding of an autologous protein, which causes its local or systemic deposition in an aberrant fibrillar form. It is quite rare for pulmonary tissue to be impacted by amyloidosis; of the three forms it can take when involving pulmonary tissue, nodular pulmonary amyloidosis is the most uncommon. Nodular pulmonary amyloidosis rarely induces clinical symptoms, and most often, it is discovered accidentally during an autopsy or via imaging techniques. Only one case of nodular pulmonary amyloidosis, which manifested as a spontaneous pneumothorax, was found in the literature. In terms of more precise subtyping, nodular amyloidosis is typically AL or mixed AL/AH type. No publications on AH-dominant type of nodular amyloidosis were found in the literature. We present a case of an 81 years-old male with nodular pulmonary AH-dominant type amyloidosis who presented with spontaneous pneumothorax. For a deeper understanding of the subject, this study also provides a review of the literature on cases with nodular pulmonary amyloidosis in relation to precise amyloid fibril subtyping. Since it is often a difficult process, accurate amyloid type identification is rarely accomplished. However, this information is very helpful for identifying the underlying disease process (if any) and outlining the subsequent diagnostic and treatment steps. Even so, it is crucial to be aware of this unit and make sure it is taken into consideration when making a differential diagnosis of pulmonary lesions.

• MeSH
• Amyloidosis * complications   diagnosis   pathology   MeSH
• Humans MeSH
• Lung diagnostic imaging   pathology   MeSH
• Lung Diseases * complications   diagnosis   pathology   MeSH
• Pneumothorax * diagnosis   etiology   MeSH
• Aged, 80 and over MeSH
• Check Tag
• Humans MeSH
• Male MeSH
• Aged, 80 and over MeSH
• Publication type
• Case Reports MeSH
• Review MeSH

Bolest na hrudi je jedním z nejčastějších příznaků, se kterými se setkávají lékaři pracující na akutních, interních i plicních ambulancích. Nejčastějšími situacemi, které mohou způsobovat bolesti na hrudi s důrazem na jejich diagnostiku a časnou léčbu, jsou hrudní infekce, pneumotorax, pneumomediastinum a nádory postihující pohrudniční dutinu.

Chest pain is one of the most common symptoms encountered by doctors working in acute, internal, and pulmonary clinics. In the United States in 2006, 10 percent of all visits to the emergency room were caused by diseases of the respiratory system, and chest pain was the most common symptom. The most common situations that can cause chest pain with an emphasis on their diagnosis and early treatment are chest infections, pneumothorax, pneumomediastinum and tumors affecting the pleural cavity.

• MeSH
• Chest Pain * diagnosis   etiology   MeSH
• Humans MeSH
• Mediastinal Emphysema diagnosis   pathology   therapy   MeSH
• Pleural Neoplasms diagnosis   therapy   MeSH
• Pneumonia diagnosis   etiology   therapy   MeSH
• Pneumothorax diagnosis   etiology   therapy   MeSH
• Check Tag
• Humans MeSH
• Publication type
• Review MeSH

Spontaneous pneumothorax is a serious and life-threatening complication of SARS-CoV-2 pneumonia. It most commonly occurs during the acute phase of the disease in patients with pre-existing lung disease (e.g. emphysema, bronchiectasis, cystic fibrosis, etc.) and in patients who require oxygen supplementation in any form (low-flow oxygen therapy, high-flow non-invasive or mechanical invasive or mechanical invasion). A rare case of a 52-year-old patient with a spontaneous pneumothorax who developed four weeks after PCR SARS-CoV-2 positivity was described. Interestingly, the patient did not have any factors that the literature considered risky for the development of this complication. During the acute phase of the disease, his condition did not require hospitalization. Imaging examinations could not clarify the cause of pneumothorax. With this case report, we want to point out the fact that spontaneous pneumothorax, as a rare and life-threatening complication of COVID-19 infection, may develop during recovery, and it is necessary to think about this complication in the differential diagnosis of dyspnoea.

• MeSH
• COVID-19 * complications   MeSH
• Middle Aged MeSH
• Humans MeSH
• Pneumothorax * diagnosis   etiology   therapy   MeSH
• SARS-CoV-2 MeSH
• Check Tag
• Middle Aged MeSH
• Humans MeSH
• Publication type
• Journal Article MeSH
• Case Reports MeSH

• MeSH
• Chest Pain diagnosis   etiology   pathology   MeSH
• Diagnosis, Differential MeSH
• Child * MeSH
• Humans MeSH
• Pleural Effusion diagnosis   complications   physiopathology   therapy   MeSH
• Lung * pathology   MeSH
• Pulmonary Embolism diagnosis   epidemiology   etiology   pathology   MeSH
• Pneumothorax diagnosis   classification   pathology   therapy   MeSH
• Check Tag
• Child * MeSH
• Humans MeSH
• Publication type
• Review MeSH

Pneumotorax je málo častou, ale zato leckdy fatální komplikací covid-19 pneumonie. Ohroženi jsou zejména muži nad 47,5–55 let s těžkým a kritickým průběhem covid-19 pneumonie, převážně na mechanické ventilaci, která však není nutnou podmínkou. Management pneumotoraxu je obdobný jako u pneumotoraxů jiné etiologie, stěžejní roli hraje hrudní drenáž, s maximální snahou o vyhnutí se nutnosti chirurgické intervence. V případech tenzního pneumotoraxu může být nutná jako provizorium akutní hrudní dekomprese jehlou, která může být život zachraňujícím výkonem. V dispenzarizaci pacientů po covid-19 pneumonii je nutno myslet na možnost pozdního rozvoje pneumotoraxu, zejména u pacientů s pneumatokélami. Tato kazuistika prezentuje případ vzniku spontánního tenzního pneumotoraxu jako pozdní komplikace covid-19 pneumonie.

Pneumothorax is an infrequent, yet often fatal, complication of COVID-19 pneumonia. Men over 47.5–55 years of age with severe to critical course of COVID-19 pneumonia are at the highest risk. Despite the majority of the incidence being linked to mechanical ventilation, this complication may appear even in spontaneously breathing patients. The management of the condition is analogous to pneumothoraces of other aetiology with chest drainage playing the most important role. Efforts are made to avoid the necessity of performing thoracic surgery. In case of tension pneumothorax, acute chest decompression via needle may be necessary as a temporary, yet sometimes life-saving, solution. In the follow-up of post-COVID-19 patients, it is important to consider the possibility of a pneumothorax as a late complication of pneumonia, especially in patients with pneumatocele. This report presents a case of a spontaneous pneumothorax as a late complication of COVID-19 pneumonia.

• MeSH
• COVID-19 * complications   MeSH
• Adult MeSH
• Humans MeSH
• Pleurodesis MeSH
• Pneumothorax * diagnosis   complications   therapy   MeSH
• Radiography, Thoracic MeSH
• Thoracentesis methods   adverse effects   MeSH
• Treatment Outcome MeSH
• Check Tag
• Adult MeSH
• Humans MeSH
• Male MeSH
• Publication type
• Case Reports MeSH

• MeSH
• Exudates and Transudates chemistry   MeSH
• Clinical Laboratory Techniques MeSH
• Humans MeSH
• Mesothelioma diagnosis   classification   therapy   MeSH
• Pleural Diseases * diagnosis   etiology   classification   therapy   MeSH
• Pleural Cavity pathology   MeSH
• Pleural Effusion diagnosis   epidemiology   etiology   classification   physiopathology   therapy   MeSH
• Pleurisy diagnosis   etiology   classification   MeSH
• Pneumothorax diagnosis   etiology   classification   MeSH
• Check Tag
• Humans MeSH
• Publication type
• Review MeSH