Bisphenols disrupt differentiation of the pigmented cells during larval brain formation in the ascidian
Jazyk angličtina Země Nizozemsko Médium print-electronic
Typ dokumentu časopisecké články
PubMed
31561137
DOI
10.1016/j.aquatox.2019.105314
PII: S0166-445X(19)30576-4
Knihovny.cz E-zdroje
- Klíčová slova
- Ascidian, Bisphenol A, Estrogen-related receptor, Invertebrate, Neurodevelopment, Otolith,
- MeSH
- benzhydrylové sloučeniny chemie toxicita MeSH
- buněčná diferenciace účinky léků MeSH
- chemické látky znečišťující vodu toxicita MeSH
- embryo nesavčí účinky léků MeSH
- estrogenní receptor ERRalfa MeSH
- fenoly chemie toxicita MeSH
- larva účinky léků metabolismus MeSH
- mozek cytologie embryologie MeSH
- organogeneze * účinky léků MeSH
- otolitová membrána cytologie účinky léků MeSH
- pigmentace * účinky léků MeSH
- pohyb buněk účinky léků MeSH
- receptory pro estrogeny antagonisté a inhibitory metabolismus MeSH
- testy toxicity MeSH
- Urochordata cytologie embryologie MeSH
- zvířata MeSH
- Check Tag
- zvířata MeSH
- Publikační typ
- časopisecké články MeSH
- Názvy látek
- benzhydrylové sloučeniny MeSH
- bisphenol A MeSH Prohlížeč
- chemické látky znečišťující vodu MeSH
- fenoly MeSH
- receptory pro estrogeny MeSH
The endocrine disruptor Bisphenol A (BPA), a widely employed molecule in plastics, has been shown to affect several biological processes in vertebrates, mostly via binding to nuclear receptors. Neurodevelopmental effects of BPA have been documented in vertebrates and linked to neurodevelopmental disorders, probably because some nuclear receptors are present in the vertebrate brain. Similarly, endocrine disruptors have been shown to affect neurodevelopment in marine invertebrates such as ascidians, mollusks or echinoderms, but whether invertebrate nuclear receptors are involved in the mode-of-action is largely unknown. In this study, we assessed the effect of BPA on larval brain development of the ascidian Phallusia mammillata. We found that BPA is toxic to P. mammillata embryos in a dose-dependent manner (EC50: 11.8μM; LC50: 21μM). Furthermore, micromolar doses of BPA impaired differentiation of the ascidian pigmented cells, by inhibiting otolith movement within the sensory vesicle. We further show that this phenotype is specific to other two bisphenols (BPE and BPF) over a bisphenyl (2,2 DPP). Because in vertebrates the estrogen-related receptor gamma (ERRγ) can bind bisphenols with high affinity but not bisphenyls, we tested whether the ascidian ERR participates in the neurodevelopmental phenotype induced by BPA. Interestingly, P. mammillata ERR is expressed in the larval brain, adjacent to the differentiating otolith. Furthermore, antagonists of vertebrate ERRs also inhibited the otolith movement but not pigmentation. Together our observations suggest that BPA may affect ascidian otolith differentiation by altering Pm-ERR activity whereas otolith pigmentation defects might be due to the known inhibitory effect of bisphenols on tyrosinase enzymatic activity.
Citace poskytuje Crossref.org