Chemical analyses of Fusarium avenaceum grown on banana medium resulted in eight novel spiroleptosphols, T1, T2 and U-Z (1-8). The structures were elucidated by a combination of high-resolution mass spectrometric data and 1- and 2-D NMR experiments. The relative stereochemistry was assigned by 1H coupling and NOESY/ROESY experiments. Absolute stereochemistry established for 7 by vibrational circular dichroism was found analogous to that of the putative polyketide spiroleptosphol from Leptosphaeria doliolum.

Department of Chemistry and Bioscience Aalborg University Niels Bohrs Vej 8 6700 Esbjerg Denmark

Department of Chemistry and Bioscience Section for Biotechnology Aalborg University Frederik Bajers Vej 7H 9220 Aalborg Ø Denmark

Institute of Organic Chemistry and Biochemistry Czech Academy of Sciences Flemingovo náměstí 542 2 166 10 Prague 6 Czech Republic

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