Evaluation of endothelial glycocalyx in healthy volunteers - An observational study
Jazyk angličtina Země Spojené státy americké Médium print
Typ dokumentu časopisecké články, pozorovací studie
PubMed
31683466
DOI
10.3233/ch-190581
PII: CH190581
Knihovny.cz E-zdroje
- Klíčová slova
- Endothelial glycocalyx, healthy volunteers, syndecan-1,
- MeSH
- biologické markery metabolismus MeSH
- dospělí MeSH
- endoteliální buňky metabolismus MeSH
- glykokalyx metabolismus MeSH
- lidé MeSH
- mladý dospělý MeSH
- zdraví dobrovolníci pro lékařské studie MeSH
- Check Tag
- dospělí MeSH
- lidé MeSH
- mladý dospělý MeSH
- mužské pohlaví MeSH
- ženské pohlaví MeSH
- Publikační typ
- časopisecké články MeSH
- pozorovací studie MeSH
- Názvy látek
- biologické markery MeSH
BACKGROUND: Endothelial glycocalyx (EG) is a carbohydrate-rich gel-like mesh covering the apical surface of endothelial cells. It has been linked to the microvascular pathophysiology and tissue metabolism. However, little is known about its condition in young healthy adults. OBJECTIVE: We aimed to describe the condition of EG in young healthy adults by in vivo EG imaging and measurement of syndecan-1, a plasma marker of EG integrity in order to obtain reference values. METHODS: For in vivo EG studies we used Side-stream Dark Field imaging of the sublingual microcirculation. Recordings were analysed automatically by GlycoCheck software providing the Perfused Boundary Region (PBR) as a marker of EG thickness. Levels of syndecan-1 were analysed in plasma samples by ELISA. RESULTS: 21 volunteers were included in the study. Median of the PBR value was 1.82 μm (interquartile range 1.69-2.01, 95% CI 1.79-1.97). Median concentration of syndecan-1 was 0.3 ng/ml (interquartile range 0.23-0.39, 95% CI 0.27-0.49). CONCLUSION: This study provides a comparison for cohorts of patients with a particular disease where the EG is presumably damaged. Our findings do not entirely comply with already published data in healthy individuals.
Charles University Faculty of Medicine in Hradec Kralove Hradec Kralove Czech Republic
Department of Microbiology and Immunology Dalhousie University Halifax NS Canada
Department of Pharmacology Dalhousie University Halifax NS Canada
Department of Physiology and Biophysics Dalhousie University Halifax NS Canada
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