CE determination of the thermodynamic pKa values and limiting ionic mobilities of 14 low molecular mass UV absorbing ampholytes for accurate characterization of the pH gradient in carrier ampholytes-based IEF and its numeric simulation
Jazyk angličtina Země Německo Médium print-electronic
Typ dokumentu časopisecké články, práce podpořená grantem
Grantová podpora
Research Gift No. 4135
Agilent Foundation - International
Grant No. 18-11776S
Grantová Agentura České Republiky - International
PubMed
31721266
DOI
10.1002/elps.201900381
Knihovny.cz E-zdroje
- Klíčová slova
- Capillary isoelectric focusing, Limiting ionic mobility, Thermodynamic acid dissociation constant, pH Gradient linearity, pI Marker,
- MeSH
- amfolytové směsi chemie MeSH
- elektroforéza kapilární metody MeSH
- isoelektrická fokusace metody MeSH
- koncentrace vodíkových iontů MeSH
- osmolární koncentrace MeSH
- počítačová simulace MeSH
- pufry MeSH
- termodynamika MeSH
- Publikační typ
- časopisecké články MeSH
- práce podpořená grantem MeSH
- Názvy látek
- amfolytové směsi MeSH
- pufry MeSH
Fourteen low molecular mass UV absorbing ampholytes containing 1 or 2 weakly acidic and 1 or 2 weakly basic functional groups that best satisfy Rilbe's requirement for being good carrier ampholytes (ΔpKa = pKamonoanion - pKamonocation < 2) were selected from a large group of commercially readily available ampholytes in a computational study using two software packages (ChemSketch and SPARC). Their electrophoretic mobilities were measured in 10 mM ionic strength BGEs covering the 2 < pH < 12 range. Using our Debye-Hückel and Onsager-Fuoss laws-based new software, AnglerFish (freeware, https://echmet.natur.cuni.cz/software/download), the effective mobilities were recalculated to zero ionic strength from which the thermodynamic pKa values and limiting ionic mobilities of the ampholytes were directly calculated by Henderson-Hasselbalch equation-type nonlinear regression. The tabulated thermodynamic pKa values and limiting ionic mobilities of these ampholytes (pI markers) facilitate both the overall and the narrow-segment characterization of the pH gradients obtained in IEF in order to mitigate the errors of analyte ampholyte pI assignments caused by the usual (but rarely proven) assumption of pH gradient linearity. These thermodynamic pKa and limiting mobility values also enable the reality-based numeric simulation of the IEF process using, for example, Simul (freeware, https://echmet.natur.cuni.cz/software/download).
Agilent Technologies Deutschland GmbH Waldbronn Germany
Chemistry Department Texas A and M University College Station TX USA
Zobrazit více v PubMed
Rilbe, H., Ann. N. Y. Acad. Sci. 1973, 209, 11-22.
Rilbe, H., Electrophoresis 1984, 5, 1-17.
Frederiksson, S., Anal. Biochem. 1972, 70, 575-585.
Righetti, P. G., Wenisch, E., Faupel, M., J. Chromaotgr. 1989, 475, 293-307.
Weber, G., Boček, P., Electrophoresis 1996, 17, 1896-1910.
Shimura, K., Wang, Z., Matsumoto, H., Kasai, K., Electrophoresis 2000, 21, 603-610.
Šlais, K., Friedl, Z., J. Chromatogr. A 1994, 661, 249-256.
Righetti, P. G., Gianazza, E., J. Chromatogr. 1977, 137, 171-181.
Šlais, K., Friedl, Z., J. Chromatogr. A 1995, 695, 113-122.
Caslavska, J., Molteni, S., Chmelik, J., Šlais, K., Matulik, F., Thormann, W., J. Chromatogr. A 1995, 680, 549-559.
Friedl, Z., Šlais, K., Chem. Listy 1997, 91, 679-680.
Šlais, K., Horká, M., Nováčková, J., Friedl, Z., Electrophoresis 2002, 23, 1682-1688.
Šťastná, M., Trávníček, M., Šlais, K., Electrophoresis 2005, 26, 53-59.
Shimura, K., Kamiya, K., Matsumoto, H., Kasai, K., Electrophoresis 2002, 74, 1046-1053.
Vigh, G., Li, M., US Patent 9,689,841, 2017.
Klepárník, K., Šlais, K., Boček, P., Electrophoresis 1993, 14, 475-479.
Lalwani, S., Tutu, E., Vigh, G., Electrophoresis 2005, 26, 2047-2055.
Šolínová, V., Kašička, B., Electrophoresis 2013, 34, 2655-2665.
Glukhovskiy, P. V., Vigh, G., Electrophoresis 1998, 19, 3166-3170.
Hruška, V., Jaroš, M., Gaš, B., Electrophoresis 2006, 27, 984-991.
Hruška, V., Beneš, M., Svobodová, J., Zusková, I., Gaš, B., Electrophoresis 2012, 33, 938-947.
Malý, M., Dovhunová, M., Dvořák, M., Gerlor, G. S., Kler, P. A., Hruška, V., Dubský, P., Electrophoresis 2019, 40, 683-692.
Tahupi, Y., Schmidt, D. E., Lindner, W., Karger, B. L., Anal. Biochem. 1981, 115, 123-129.
Katayama, H., Ishihama, Y., Asakawa, N., Anal. Chem. 1998, 70, 5272-5277.
North, R., Vigh, G., Electrophoresis 2008, 29, 1077-1081.
Lalwani, S., Tutu, E., Vigh, G., Electrophoresis 2005, 26, 2503-2510.
Cai, J., Smith, J. T., J. High Resolut. Chromatogr. 1992, 15, 30-32.
Malý, M., Boublík, M., Pocrnić, M., Ansorge, M., Lorinčíková, K., Svobodová, J., Hruška, V., Dubský, P., Gaš, B., Electrophoresis 2020, 41, https://doi.org/10.1002/elps.201900283.
Debye, P., Hückel, E., Physik. Z. 1923, 24, 185-206.
Debye, P., Hückel, E., Physik. Z. 1923, 24, 305-325.
Davies, C. W., Ion Association, Butterworths, London, 1962.
Onsager, L., Fuoss, R. M., J. Phys. Chem. 1932, 36, 2689-2778.
Hirokawa, T., Nishimo, M., Aoki, N., Kiso, Y., Sawamoto, Y., Yagi, T., Akiyama, J., J. Chromatogr. 1983, 271, D1-D106.
Mosher, R. A., Thormann, W., Electrophoresis 1990, 11, 717-723.
Bossi, A., Righetti, P. G, Electrophoresis 1997, 18, 2012-2018.
Fitch, C. A., Platzer, G., Okon, M., Garcia-Moreno, B., McIntosh L. P., Protein Sci. 2015, 24, 752-761.
