miR-181a-2* expression is different amongst carcinomas from the colorectal serrated route
Jazyk angličtina Země Velká Británie, Anglie Médium print
Typ dokumentu časopisecké články, práce podpořená grantem
PubMed
31784758
DOI
10.1093/mutage/gez039
PII: 5648152
Knihovny.cz E-zdroje
- MeSH
- CpG ostrůvky MeSH
- cytokiny genetika metabolismus MeSH
- faktor 1 asociovaný s receptory TNF genetika metabolismus MeSH
- genová ontologie MeSH
- karcinom genetika metabolismus patofyziologie MeSH
- kolorektální nádory genetika metabolismus patofyziologie MeSH
- lidé MeSH
- metylace DNA MeSH
- mikro RNA genetika metabolismus MeSH
- mikrosatelitní nestabilita * MeSH
- nádorové buněčné linie MeSH
- nikotinamidfosforibosyltransferasa genetika metabolismus MeSH
- regulace genové exprese u nádorů genetika MeSH
- sekvenční analýza hybridizací s uspořádaným souborem oligonukleotidů MeSH
- senioři MeSH
- transkripční faktory genetika metabolismus MeSH
- Check Tag
- lidé MeSH
- mužské pohlaví MeSH
- senioři MeSH
- ženské pohlaví MeSH
- Publikační typ
- časopisecké články MeSH
- práce podpořená grantem MeSH
- Názvy látek
- cytokiny MeSH
- faktor 1 asociovaný s receptory TNF MeSH
- mikro RNA MeSH
- MIrn181 microRNA, human MeSH Prohlížeč
- nicotinamide phosphoribosyltransferase, human MeSH Prohlížeč
- nikotinamidfosforibosyltransferasa MeSH
- SALL1 protein, human MeSH Prohlížeč
- transkripční faktory MeSH
Serrated adenocarcinoma (SAC) and colorectal carcinomas showing histological and molecular features of high-level of microsatellite instability (hmMSI-H) are both end points of the serrated pathway of colorectal carcinogenesis. Despite common features (right-sided location, CpG island methylation phenotype and BRAF mutation) there are no studies comparing the microRNA (miRNA) expression profiles in SACs and hmMSI-H. The microtranscriptome from 12 SACs and 8 hmMSI-H were analysed using Affymetrix GeneChip miRNA 3.0 arrays and differentially enriched functions involving immune response were observed from this comparison. miR-181a-2* was found significantly more expressed in hmMSI-H than in SAC and higher expression of this miRNA in microsatellite unstable colorectal cancer were corroborated by Real-Time PCR in an extended series (61 SAC, 21 hmMSI-H). An analysis of genes possibly regulated by miR-181a-2* was carried out and, amongst these, an inverse correlation of NAMPT with miR-181a-2* expression was observed, whereas, for TRAF1 and SALL1, additional regulation mechanisms involving CpG island methylation were observed. miR-181a-2* is associated with particular histological and molecular features of colorectal carcinomas within the serrated pathological pathway and might play a role in the immune responses of microsatellite instability carcinomas.
Central European Institute of Technology Masaryk University Brno Czech Republic
Clinical Analysis Department Santa Lucia University Hospital Cartagena Spain
Department of Surgery University Hospital Brno Brno Bohunice Brno Starý Lískovec Czech Republic
Pathology Department Santa Lucia University Hospital Cartagena Spain
Citace poskytuje Crossref.org
