Plasmid DNA is commonly used as a simpler substitute for a cell in studies of early effects of ionizing radiation because it allows to determine yields of primary DNA lesions. Experimental studies often employ plasmids of different lengths, in different concentrations in the aqueous solution. Influence of these parameters on the heavy-ion induced yields of primary DNA damage has been studied, using plasmids pUC19 (2686 bp), pBR322 (4361 bp) and pKLAC2 (9107 bp) in 10 and 50 ng/μl concentration. Results demonstrate the impact of plasmid length, while no significant difference was observed between the two concentrations. The uncertainty of the results is discussed.

Department of Radiation Dosimetry Nuclear Physics Institute of the CAS Na Truhlářce 39 64 180 00 Praha Czech Republic

Faculty of Nuclear Sciences and Physical Engineering Czech Technical University Prague Břehová 78 7 110 00 Praha Czech Republic

National Institute of Radiological Sciences 4 9 1 Anagawa Inage 263 8555 Chiba Japan

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