RADIATION-INDUCED PLASMID DNA DAMAGE: EFFECT OF CONCENTRATION AND LENGTH
Language English Country England, Great Britain Media print
Document type Journal Article
PubMed
31803909
DOI
10.1093/rpd/ncz196
PII: 5658464
Knihovny.cz E-resources
- MeSH
- Algorithms MeSH
- Antioxidants pharmacology MeSH
- DNA radiation effects MeSH
- Radiation, Ionizing MeSH
- Linear Energy Transfer MeSH
- Monte Carlo Method MeSH
- Uncertainty MeSH
- Plasmids radiation effects MeSH
- Polypropylenes chemistry MeSH
- DNA Damage radiation effects MeSH
- Heavy Ions MeSH
- Dose-Response Relationship, Radiation MeSH
- Gamma Rays MeSH
- Publication type
- Journal Article MeSH
- Names of Substances
- Antioxidants MeSH
- DNA MeSH
- Polypropylenes MeSH
Plasmid DNA is commonly used as a simpler substitute for a cell in studies of early effects of ionizing radiation because it allows to determine yields of primary DNA lesions. Experimental studies often employ plasmids of different lengths, in different concentrations in the aqueous solution. Influence of these parameters on the heavy-ion induced yields of primary DNA damage has been studied, using plasmids pUC19 (2686 bp), pBR322 (4361 bp) and pKLAC2 (9107 bp) in 10 and 50 ng/μl concentration. Results demonstrate the impact of plasmid length, while no significant difference was observed between the two concentrations. The uncertainty of the results is discussed.
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