Understorey plant communities are crucial to maintain species diversity and ecosystem processes including nutrient cycling and regeneration of overstorey trees. Most studies exploring effects of elevated CO2 concentration ([CO2]) in forests have, however, been done on overstorey trees, while understorey communities received only limited attention.The hypothesis that understorey grass species differ in shade-tolerance and development dynamics, and temporally exploit different niches under elevated [CO2], was tested during the fourth year of [CO2] treatment. We assumed stimulated carbon gain by elevated [CO2] even at low light conditions in strongly shade-tolerant Luzula sylvatica, while its stimulation under elevated [CO2] in less shade-tolerant Calamagrostis arundinacea was expected only in early spring when the tree canopy is not fully developed.We found evidence supporting this hypothesis. While elevated [CO2] stimulated photosynthesis in L. sylvatica mainly in the peak of the growing season (by 55%-57% in July and August), even at low light intensities (50 µmol m-2 s-1), stimulatory effect of [CO2] in C. arundinacea was found mainly under high light intensities (200 µmol m-2 s-1) at the beginning of the growing season (increase by 171% in May) and gradually declined during the season. Elevated [CO2] also substantially stimulated leaf mass area and root-to-shoot ratio in L. sylvatica, while only insignificant increases were observed in C. arundinacea.Our physiological and morphological analyses indicate that understorey species, differing in shade-tolerance, under elevated [CO2] exploit distinct niches in light environment given by the dynamics of the tree canopy.

Global Change Research Institute of the Czech Academy of Sciences Brno Czech Republic

Southern Swedish Forest Research Centre Swedish University of Agricultural Sciences Alnarp Sweden

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