Methods to assess DC-dependent priming of T cell responses by dying cells
Jazyk angličtina Země Spojené státy americké Médium print-electronic
Typ dokumentu časopisecké články, práce podpořená grantem
PubMed
32000914
DOI
10.1016/bs.mie.2019.05.045
PII: S0076-6879(19)30226-5
Knihovny.cz E-zdroje
- Klíčová slova
- Anti-tumor immune response, Generation of dendritic cells, Phagocytosis, T cell activation,
- MeSH
- aktivace lymfocytů * MeSH
- apoptóza MeSH
- dendritické buňky imunologie MeSH
- fagocytóza MeSH
- kultivované buňky MeSH
- lidé MeSH
- nádory imunologie MeSH
- průtoková cytometrie metody MeSH
- T-lymfocyty imunologie MeSH
- zvířata MeSH
- Check Tag
- lidé MeSH
- zvířata MeSH
- Publikační typ
- časopisecké články MeSH
- práce podpořená grantem MeSH
Dendritic cells have been widely investigated in cancer immunotherapy clinical trials for the last two decades mainly due to their robust ability to elicit an adaptive anticancer immune response of the cellular and humoral types. Immature DCs can be easily loaded with desired antigens. However, to become efficient antigen-presenting cells, DCs must first undergo a process of maturation. Protocols for the generation of DCs for use in cancer immunotherapy, including the generation of a large number of immature DCs for antigen pulsing and the selection of a well-defined immunostimulatory agent to achieve complete and reproducible maturation, which is a crucial step for further stimulation of T cell activation, must carefully consider the characteristics of DC physiology. In this report, we provided a detailed protocol for DC generation, pulsation and activation with the subsequent induction of T cell-specific immune responses.
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