We recently developed a new light source that allows for the continuous monitoring of light-induced changes using common spectrophotometric devices adapted for microplate analyses. This source was designed primarily to induce photodynamic processes in cell models. Modern light components, such as LED chips, were used to improve the irradiance homogeneity. In addition, this source forms a small hermetic chamber and thus allows for the regulation of the surrounding atmosphere, which plays a significant role in these light-dependent reactions. The efficacy of the new light source was proven via kinetic measurements of reactive oxygen species generated during the photodynamic reaction of chloroaluminium phthalocyanine disulfonate (ClAlPcS2) in three cell lines: human melanoma cells (G361), human breast adenocarcinoma cells (MCF7), and human fibroblasts (BJ).

Department of Medical Biophysics Faculty of Medicine and Dentistry Palacky University in Olomouc Hnevotinska 3 775 15 Olomouc Czech Republic

Faculty of Medicine and Dentistry Institute of Molecular and Translational Medicine Palacky University in Olomouc Hnevotinska 3 775 15 Olomouc Czech Republic

Regional Innovation Centre for Electrical Engineering University of West Bohemia Univerzitni 26 306 14 Pilsen Czech Republic

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