The application of antimicrobial photodynamic therapy on S. aureus and E. coli using porphyrin photosensitizers bound to cyclodextrin
Jazyk angličtina Země Německo Médium print-electronic
Typ dokumentu časopisecké články, práce podpořená grantem
PubMed
23899404
DOI
10.1016/j.micres.2013.07.005
PII: S0944-5013(13)00114-6
Knihovny.cz E-zdroje
- Klíčová slova
- Antimicrobial photodynamic therapy, Gram-negative bacteria, Gram-positive bacteria, Photodynamic therapy, Porphyrins,
- MeSH
- buněčné linie MeSH
- cyklodextriny chemie farmakologie MeSH
- Escherichia coli účinky léků účinky záření MeSH
- fotochemoterapie MeSH
- fotosenzibilizující látky chemie farmakologie MeSH
- infekce vyvolané Escherichia coli farmakoterapie mikrobiologie MeSH
- lidé MeSH
- porfyriny chemie farmakologie MeSH
- stafylokokové infekce farmakoterapie mikrobiologie MeSH
- Staphylococcus aureus účinky léků účinky záření MeSH
- světlo MeSH
- Check Tag
- lidé MeSH
- Publikační typ
- časopisecké články MeSH
- práce podpořená grantem MeSH
- Názvy látek
- cyklodextriny MeSH
- fotosenzibilizující látky MeSH
- porfyriny MeSH
Photodynamic therapy is usually used against malignant and non-malignant tumors. Nowadays, due to resistance of bacterial strains, we are looking for a new antimicrobial strategy to destroy bacteria with minimal invasive consequences. The worldwide increase in antibiotic resistance among different classes of gram-positive and gram-negative bacteria has led to the search for alternative anti-microbial therapies such as antimicrobial PDT (aPDT). Development antimicrobial technology combines a nontoxic compound, called photosensitizer, visible light of the appropriate wavelength, and the generation of reactive oxygen species. In this work, the photosensitizers TMPyP and ZnTPPS4 are investigated for photodynamic and antimicrobial photodynamic therapy. We tested these two porphyrins on two cell lines and two bacterial strains to compare effectiveness. In addition, we applied photosensitizers bound in the complex created with hp-β-cyclodextrin. The light-emitting diodes were used at the doses 0, 1, 5, 10 J/cm(2) for cells and 0, 150 J/cm(2) for bacteria. Tested concentrations for cells and microbes were from 0.5 to 50 μM and from 0.78 to 100 μM, respectively. From this work it can be concluded that TMPyP is a promising compound both in aPDT and in PDT, particularly in contrast to ZnTPPS4, which was efficient only in PDT. Furthermore, the eradication of gram-positive bacteria is possible only with higher concentrations of ZnTPPS4.
Citace poskytuje Crossref.org
New planar light source for the induction and monitoring of photodynamic processes in vitro
