The design and application of an inkjet-printed electrochemically reduced graphene oxide microelectrode for HT-2 mycotoxin immunoenzymatic biosensing is reported. A water-based graphene oxide ink was first formulated and single-drop line working microelectrodes were inkjet-printed onto poly(ethylene 2,6-naphthalate) substrates, with dimensions of 78 μm in width and 30 nm in height after solvent evaporation. The printed graphene oxide microelectrodes were electrochemically reduced and characterized by Raman and X-ray photoelectron spectroscopies in addition to microscopies. Through optimization of the electrochemical reduction parameters, differential pulse voltammetry were performed to examine the sensing of 1-naphthol (1-N), where it was revealed that reduction times had significant effects on electrode performance. The developed microelectrodes were then used as an immunoenzymatic biosensor for the detection of HT-2 mycotoxin based on carbodiimide linking of the microelectrode surface and HT-2 toxin antigen binding fragment of antibody (anti-HT2 (10) Fab). The HT-2 toxin and anti-HT2 (10) Fab reaction was reported by anti-HT2 immune complex single-chain variable fragment of antibody fused with alkaline phosphatase (anti-IC-HT2 scFv-ALP) which is able to produce an electroactive reporter - 1-N. The biosensor showed detection limit of 1.6 ng ∙ mL-1 and a linear dynamic range of 6.3 - 100.0 ng ∙ mL-1 within a 5 min incubation with 1-naphthyl phosphate (1-NP) substrate.

Catalan Institute of Nanoscience and Nanotechnology CSIC and The Barcelona Institute of Science and Technology Bellaterra ES 08193 Spain

Catalan Institute of Nanoscience and Nanotechnology CSIC and The Barcelona Institute of Science and Technology Bellaterra ES 08193 Spain; Department of Chemical Engineering School of Engineering UAB Bellaterra ES 08193 Spain

Catalan Institute of Nanoscience and Nanotechnology CSIC and The Barcelona Institute of Science and Technology Bellaterra ES 08193 Spain; Department of Chemistry and Biochemistry Mendel University in Brno Zemedelska 1 Brno CZ 613 00 Czech Republic

Catalan Institute of Nanoscience and Nanotechnology CSIC and The Barcelona Institute of Science and Technology Bellaterra ES 08193 Spain; ICREA Institució Catalana de Recerca i Estudis Avançats Barcelona ES 08010 Spain

Department of Chemistry and Biochemistry Mendel University in Brno Zemedelska 1 Brno CZ 613 00 Czech Republic; Central European Institute of Technology Brno University of Technology Brno CZ 616 00 Czech Republic

VTT Technical Research Centre of Finland Espoo FI 02150 Finland

Citace poskytuje Crossref.org

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