This study presents an innovative multifunctional system in fabricating new functional wound dressing (FWD) products that could be used for skin regeneration, especially in cases of infected chronic wounds and ulcers. The innovation is based on the extraction, characterization, and application of collagen (CO)/chitosan-glucan complex hollow fibers (CSGC)/aloe vera (AV) as a novel FWS. For the first time, specific hollow fibers were extracted with controlled inner (500-900 nm)/outer (2-3 µm) diameters from mycelium of Schizophyllum commune. Further on, research and evaluation of morphology, hydrolytic stability, and swelling characteristics of CO/CSGC@AV were carried out. The obtained FWS showed high hydrolytic stability with enhanced swelling characteristics compared to native collagen. The hemostatic effect of FWS increased significantly in the presence of CSGC, compared to native CO and displayed excellent biocompatibility which was tested by using normal human dermal fibroblast (NHDF). The FWS showed high antibacterial activity against different types of bacteria (positive/negative grams). From in vivo measurements, the novel FWS increased the percentage of wound closure after one week of treatment. All these results imply that the new CO/CSGC@AV-FWD has the potential for clinical skin regeneration and applying for controlled drug release.

CEITEC Central European Institute of Technology Brno University of Technology Purkyňova 656 123 Brno 612 00 Czechia

CEITEC Central European Institute of Technology Brno University of Technology Purkyňova 656 123 Brno 612 00 Czechia; SCITEG a s Brno Czechia; Institute of Materials Chemistry Facility of Chemistry Brno University of Technology Purkyňova 464 118 Brno 612 00 Czechia

CEITEC Central European Institute of Technology Brno University of Technology Purkyňova 656 123 Brno 612 00 Czechia; SCITEG a s Brno Czechia; Pretreatment and Finishing of Cellulosic based Textiles Department Textile Industries Research Division National Research Centre 33 EL Buhouth St Dokki Giza 12622 Egypt

Department of Medical Chemistry and Biochemistry Faculty of Medicine and Dentistry Palacky University Hněvotínská 3 775 15 Olomouc Czechia

Institute of Physics of Materials Academy of Sciences of the Czech Republic Žižkova 22 CZ 61662 Brno Czechia

Pathology Department National Research Centre 33 EL Buhouth St Dokki Giza 12622 Egypt

Pharmacology Department National Research Centre 33 EL Buhouth St Dokki Giza 12622 Egypt

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