Peptides derived from non-functional precursors play important roles in various developmental processes, but also in (a)biotic stress signaling. Our (phospho)proteome-wide analyses of C-TERMINALLY ENCODED PEPTIDE 5 (CEP5)-mediated changes revealed an impact on abiotic stress-related processes. Drought has a dramatic impact on plant growth, development and reproduction, and the plant hormone auxin plays a role in drought responses. Our genetic, physiological, biochemical, and pharmacological results demonstrated that CEP5-mediated signaling is relevant for osmotic and drought stress tolerance in Arabidopsis, and that CEP5 specifically counteracts auxin effects. Specifically, we found that CEP5 signaling stabilizes AUX/IAA transcriptional repressors, suggesting the existence of a novel peptide-dependent control mechanism that tunes auxin signaling. These observations align with the recently described role of AUX/IAAs in stress tolerance and provide a novel role for CEP5 in osmotic and drought stress tolerance.

Centre for Plant Integrative Biology University of Nottingham Loughborough United Kingdom

Centre for Plant Sciences Faculty of Biological Sciences University of Leeds Leeds United Kingdom

Department of Biology University of Washington Seattle Washington USA

Department of Life Sciences POSTECH Biotech Center Pohang University of Science and Technology Pohang Republic of Korea

Department of Plant Biotechnology and Bioinformatics Ghent University Belgium; VIB Center for Plant Systems Biology Ghent Belgium

Department of Plant Biotechnology and Bioinformatics Ghent University Belgium; VIB Center for Plant Systems Biology Ghent Belgium; VIB UGent Center for Medical Biotechnology Ghent Belgium; Department of Biomolecular Medicine Ghent University Ghent Belgium

Division of Plant and Crop Sciences School of Biosciences University of Nottingham Loughborough United Kingdom

Division of Plant and Crop Sciences School of Biosciences University of Nottingham Loughborough United Kingdom; Centre for Plant Integrative Biology University of Nottingham Loughborough United Kingdom

Division of Plant and Crop Sciences School of Biosciences University of Nottingham Loughborough United Kingdom; Department of Plant Biotechnology and Bioinformatics Ghent University Belgium; VIB Center for Plant Systems Biology Ghent Belgium; Centre for Plant Integrative Biology University of Nottingham Loughborough United Kingdom

Institute for Developmental Genetics Heinrich Heine University Düsseldorf Germany

Mendel Centre for Plant Genomics and Proteomics Central European Institute of Technology Klosterneuburg Austria

Screening Core Gent Belgium; Expertise Centre for Bioassay Development and Screening Ghent University Ghent Belgium

Umeå Plant Science Centre Department of Forest Genetics and Plant Physiology Swedish University of Agricultural Sciences Umeå Sweden

VIB UGent Center for Medical Biotechnology Ghent Belgium; Department of Biomolecular Medicine Ghent University Ghent Belgium

Zentrum für Molekularbiologie der Pflanzen Plant Biochemistry University Tübingen Tübingen Germany

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